T4393
Trombina
lyophilized powder, 1500-3500 NIH units/mg protein (E1%/280, 18.3), suitable for cell culture
Sinônimo(s):
Fator IIa
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About This Item
Produtos recomendados
fonte biológica
human plasma
Nível de qualidade
esterilidade
sterile
forma
lyophilized powder
atividade específica
1500-3500 NIH units/mg protein (E1%/280, 18.3)
técnica(s)
cell culture | mammalian: suitable
Impurezas
HIV, hepatitis B and hepatitis C, tested negative
nº de adesão UniProt
temperatura de armazenamento
−20°C
Informações sobre genes
human ... F2(2147)
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Descrição geral
Thrombin is produced from the proteolytic cleavage of inactive prothrombin in the liver. The prothrombin gene is mapped to human chromosome 11p11.2. It comprises of A and B catalytic domain, recognition domain and insertion loops. The active site residues comprise the catalytic tetrad, (histidine 57, aspartate 102, serine 195 and serine 214).
Aplicação
Thrombin from human plasma has been used:
- as a medium supplement for the pre-treatment of endothelial cell culture prior to confocal microscopy and enzyme linked immunosorbent assay (ELISA)
- in the gelatinization of mesenchymal stem cells (MSCs) for preparing fibrin–MSC construct
- for screening serine protease inhibitor, OGTI from frog skin secretion
Ações bioquímicas/fisiológicas
The main function of thrombin is the cleavage of fibrinogen to fibrin, to assist stable clot formation. High levels of thrombin elicit neurotoxicity in dopaminergic neurons and contributes to the progression of Parkinson′s disease. A wide range of mutations in the prothrombin gene contributes to its deficiency resulting in coagulation disorders like dysprothrombinemia and hypoprothrombinemia. Altered thrombin levels modulates the coagulation pathway in multiple sclerosis. Patients with coronary artery disease (CAD) show elevated levels of thrombin. Thrombin accumulation in neurofibrillary tangles in the brain may contribute to the aggregation of τ protein and pathophysiology of Alzheimer disease.
Protease serina que cliva seletivamente ligações Arg-Gly do fibrinogênio para formar fibrina e fibrinopeptídeos A e B.
Definição da unidade
Activity is expressed in NIH units obtained by direct comparison to a NIH Thrombin Reference Standard
Reconstituição
When reconstituted with 1 mL water, vial contains stated activity in 0.15 M sodium chloride and 0.05 M sodium citrate, pH 6.5.
Nota de análise
The NIH assay procedure uses 0.2 ml diluted plasma (1:1 with saline) as a substrate and 0.1ml of thrombin sample (stabilized in a 1% buffered albumin solution) based on a modification of the method of Biggs. Only clotting times in the range of 15-25 seconds are used for determining thrombin concentrations.
Exoneração de responsabilidade
RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.
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Nº do produto
Descrição
Preços
Palavra indicadora
Danger
Frases de perigo
Declarações de precaução
Classificações de perigo
Resp. Sens. 1
Código de classe de armazenamento
10 - Combustible liquids
Classe de risco de água (WGK)
WGK 3
Ponto de fulgor (°F)
Not applicable
Ponto de fulgor (°C)
Not applicable
Certificados de análise (COA)
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Artigos
Thrombin Factor IIa is an endolytic serine protease that selectively cleaves the Arg--Gly bonds of fibrinogen to form fibrin and release fibrinopeptides A and B.
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