T7009

Trombina

lyophilized powder, ≥1,000 NIH units/mg protein (E1%/280, 18.3)

• Sinônimo(s): Fator IIa
• Número CAS: 9002-04-4
• Número da licença da enzima: 3.4.21.5 (BRENDA, IUBMB)
• Número CE: 232-648-7
• Número MDL: MFCD00082072
• Código UNSPSC: 12352204
• NACRES: NA.54

Propriedades

• forma: lyophilized powder
• Nível de qualidade: 200
• atividade específica: ≥1,000 NIH units/mg protein (E1%/280, 18.3)
• peso molecular: 37.4 kDa
• Impurezas: HIV and HBsAg, source material tested negative
• nº de adesão UniProt: P00734
• aplicação(ões): diagnostic assay manufacturing
• temperatura de armazenamento: −20°C
• Informações sobre genes: human ... F2(2147)

Descrição

Descrição geral

The F2 gene encodes thrombin protein and is mapped to human chromosome 11p11.2.

Aplicação

Thrombin from human plasma has been:

• dissolved in CaC_l2 and added to poly ethylene glycolated fibrinogen/cell solution for fibrin production
• to polymerize fibrin on well plates
• used as an endogenous agonist proteinase for proteinase-activated receptors : PAR1, PAR3 and PAR4 for stimulation of cells

Ações bioquímicas/fisiológicas

Thrombin is an endolytic serine protease which stimulates vascular damage induced-leukocytes, platelets, endothelial and mesenchymal cell production. This action is mediated via G-protein-coupled receptor. It catalysis the cleavage of fibrinogen at Arg−Gly bonds to form fibrin and release fibrinopeptides A and B. The predominant form of thrombin in vivo is the zymogen prothrombin (factor II), which is produced in the liver. Prothrombin is a coagulation activation factor, high levels of which indicates cancer. Thrombin can be used to cleave fusion proteins. Cleavage of fusion proteins can be carried out at a thrombin:fusion protein ratio of 1:500.

Protease serina que cliva seletivamente ligações Arg-Gly do fibrinogênio para formar fibrina e fibrinopeptídeos A e B.

Definição da unidade

Activity is expressed in NIH units obtained by direct comparison to a NIH Thrombin Reference Standard

forma física

Lyophilized from saline sodium citrate buffer, pH 6.5

Nota de análise

The NIH assay procedure uses 0.2 ml diluted plasma (1:1 with saline) as a substrate and 0.1ml of thrombin sample (stabilized in a 1% buffered albumin solution) based on a modification of the method of Biggs. Only clotting times in the range of 15-25 seconds are used for determining thrombin concentrations.

Outras notas

View more information on thrombin at www.sigma-aldrich.com/enzymeexplorer.

Exoneração de responsabilidade

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Informações de segurança

• Pictogramas: GHS08
• Palavra indicadora: Danger
• Frases de perigo: H334
• Declarações de precaução: P261 - P284 - P501
• Classificações de perigo: Resp. Sens. 1
• Código de classe de armazenamento: 10 - Combustible liquids
• Classe de risco de água (WGK): WGK 3
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable