10602400001
Roche
Thrombin
from human plasma
Sinônimo(s):
coagulation factor Iia, thrombin
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About This Item
Produtos recomendados
fonte biológica
human plasma
Nível de qualidade
forma
lyophilized
atividade específica
~120 units/mg protein (At 25 °C with Chromozym TH as the substrate.)
peso molecular
Mr ~33.6 kDa
embalagem
pkg of 20 U
fabricante/nome comercial
Roche
pH ideal
8.2-9.0
Condições de expedição
wet ice
Descrição geral
Thrombin is a Na+ activated allosteric serine protease. It belongs to chymotrypsin family. Thrombin is made of two polypeptide chains of 36 (A chain) and 259 (B chain) residues that are covalently attached with a disulfide bond.
Thrombin is a coagulation factor IIa. It is a serine endopeptidase that hydrolyzes peptide and ester bonds specifically at the carboxylic side of Arg. The enzyme converts fibrinogen to fibrin. The product contains EDTA and additional stabilizing agents.
Aplicação
Thrombin has been used in the expression and purification of recombinant RELM (resistin-like moleculefamily members. It has also been used in platelet spreading on fibrinogen and immunostaining.
Thrombin is used in coagulation research, medical research, protein-structure analysis, and biochemical research. It has been used for the stimulation of the platelets.
Ações bioquímicas/fisiológicas
Thrombin plays an important role in blood coagulation. It serve as a procoagulant factor while transforming fibrinogen into an insoluble fibrin clot. Thrombin can act as anticoagulant by the activation of protein C.
forma física
Lyophilizate
Nota de preparo
Storage conditions (working solution): -15 to -25°C
Inhibitors: DFP, TLCK, PMSF, benzamidine, α1-antitrypsin, α2-macroglobulin, antithrombin III heparin, hirudin, and APMSF
Inhibitors: DFP, TLCK, PMSF, benzamidine, α1-antitrypsin, α2-macroglobulin, antithrombin III heparin, hirudin, and APMSF
Reconstituição
The reconstituted solution contains 20 mM EDTA.
Important note:
Use plastic vials and pipettes because thrombin will be adsorbed to glass surfaces!
Important note:
Use plastic vials and pipettes because thrombin will be adsorbed to glass surfaces!
Armazenamento e estabilidade
Store at 2 to 8 °C. (Store dry!)
Outras notas
For life science research only. Not for use in diagnostic procedures.
Exoneração de responsabilidade
RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.
Palavra indicadora
Warning
Frases de perigo
Declarações de precaução
Classificações de perigo
Acute Tox. 4 Inhalation - STOT RE 2
Código de classe de armazenamento
11 - Combustible Solids
Classe de risco de água (WGK)
WGK 2
Ponto de fulgor (°F)
does not flash
Ponto de fulgor (°C)
does not flash
Certificados de análise (COA)
Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.
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