1.12374

Thrombin

from bovine plasma, 50 NIH units/mg dry wt

• Sinônimo(s): Thrombin, Coagulation factor IIa, Fibrinogenase
• Número CAS: 9002-04-4
• Código UNSPSC: 12352204
• Número do índice EC: 232-648-7
• NACRES: NA.25

Propriedades

• fonte biológica: bovine plasma
• Nível de qualidade: 200
• forma: solid
• atividade específica: 50 NIH units/mg dry wt
• temperatura de armazenamento: 2-8°C

Descrição

Descrição geral

Thrombin, a glycosylated sodium-activated type II enzyme, is also called coagulation factor IIa. It contains two anion-binding exosites, ABE-I and ABE-II. Thrombin exists as α, β, and γ isoforms at different stages of its generation. Bovine a-thrombin consists of a light chain (A chain) and a heavy chain (B chain). These two chains are joined by one disulfide bond.

Aplicação

Thrombin has been used in flow cytometry analysis to investigate the basal activation level of circulating platelets in mice. It has also been used to coagulate the plasma and allow the organotypic slice to adhere to the coverslip during culturing.

Ações bioquímicas/fisiológicas

Thrombin is a serine protease critical in the blood clotting process and activates clotting factors V, VIII, XI, and XII. It mediates the conversion of inactive plasma protein fibrinogen (factor I) into biologically active fibrin. Thrombin acts as a pro-coagulant and promotes platelet aggregation. It can also produce anti-coagulant effects. It is used to treat bleeding from capillaries and small venules. During vascular injury, thrombin is crucial for controlling the macrophages, neutrophils, monocytes, SMC, and dendritic cells.

Nota de análise

Appearance (colour): almost white
Appearance (description): powder
Activity (fibrinogen; pH 7.6; 37 °C): ≥ 50 U/mg

Informações de segurança

• Pictogramas: GHS07,GHS08
• Palavra indicadora: Danger
• Frases de perigo: H315 - H319 - H334 - H335
• Declarações de precaução: P261 - P264 - P271 - P302 + P352 - P304 + P340 + P312 - P305 + P351 + P338
• Classificações de perigo: Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
• Órgãos-alvo: Respiratory system
• Código de classe de armazenamento: 11 - Combustible Solids
• Classe de risco de água (WGK): WGK 1
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable