Complete sequencing and characterization of 21,243 full-length human cDNAs.

Complete sequencing and characterization of 21,243 full-length human cDNAs.

Nature genetics (2004-01-01)

Toshio Ota, Yutaka Suzuki, Tetsuo Nishikawa, Tetsuji Otsuki, Tomoyasu Sugiyama, Ryotaro Irie, Ai Wakamatsu, Koji Hayashi, Hiroyuki Sato, Keiichi Nagai, Kouichi Kimura, Hiroshi Makita, Mitsuo Sekine, Masaya Obayashi, Tatsunari Nishi, Toshikazu Shibahara, Toshihiro Tanaka, Shizuko Ishii, Jun-ichi Yamamoto, Kaoru Saito, Yuri Kawai, Yuko Isono, Yoshitaka Nakamura, Kenji Nagahari, Katsuhiko Murakami, Tomohiro Yasuda, Takao Iwayanagi, Masako Wagatsuma, Akiko Shiratori, Hiroaki Sudo, Takehiko Hosoiri, Yoshiko Kaku, Hiroyo Kodaira, Hiroshi Kondo, Masanori Sugawara, Makiko Takahashi, Katsuhiro Kanda, Takahide Yokoi, Takako Furuya, Emiko Kikkawa, Yuhi Omura, Kumi Abe, Kumiko Kamihara, Naoko Katsuta, Kazuomi Sato, Machiko Tanikawa, Makoto Yamazaki, Ken Ninomiya, Tadashi Ishibashi, Hiromichi Yamashita, Katsuji Murakawa, Kiyoshi Fujimori, Hiroyuki Tanai, Manabu Kimata, Motoji Watanabe, Susumu Hiraoka, Yoshiyuki Chiba, Shinichi Ishida, Yukio Ono, Sumiyo Takiguchi, Susumu Watanabe, Makoto Yosida, Tomoko Hotuta, Junko Kusano, Keiichi Kanehori, Asako Takahashi-Fujii, Hiroto Hara, Tomo-o Tanase, Yoshiko Nomura, Sakae Togiya, Fukuyo Komai, Reiko Hara, Kazuha Takeuchi, Miho Arita, Nobuyuki Imose, Kaoru Musashino, Hisatsugu Yuuki, Atsushi Oshima, Naokazu Sasaki, Satoshi Aotsuka, Yoko Yoshikawa, Hiroshi Matsunawa, Tatsuo Ichihara, Namiko Shiohata, Sanae Sano, Shogo Moriya, Hiroko Momiyama, Noriko Satoh, Sachiko Takami, Yuko Terashima, Osamu Suzuki, Satoshi Nakagawa, Akihiro Senoh, Hiroshi Mizoguchi, Yoshihiro Goto, Fumio Shimizu, Hirokazu Wakebe, Haretsugu Hishigaki, Takeshi Watanabe, Akio Sugiyama

PMID14702039

要旨

As a base for human transcriptome and functional genomics, we created the "full-length long Japan" (FLJ) collection of sequenced human cDNAs. We determined the entire sequence of 21,243 selected clones and found that 14,490 cDNAs (10,897 clusters) were unique to the FLJ collection. About half of them (5,416) seemed to be protein-coding. Of those, 1,999 clusters had not been predicted by computational methods. The distribution of GC content of nonpredicted cDNAs had a peak at approximately 58% compared with a peak at approximately 42%for predicted cDNAs. Thus, there seems to be a slight bias against GC-rich transcripts in current gene prediction procedures. The rest of the cDNAs unique to the FLJ collection (5,481) contained no obvious open reading frames (ORFs) and thus are candidate noncoding RNAs. About one-fourth of them (1,378) showed a clear pattern of splicing. The distribution of GC content of noncoding cDNAs was narrow and had a peak at approximately 42%, relatively low compared with that of protein-coding cDNAs.

材料

製品番号

ブランド

製品内容

L1754

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リパーゼ Candida rugosa由来, Type VII, ≥700 unit/mg solid

L3126

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リパーゼブタ膵臓由来, Type II, ≥125 units/mg protein (using olive oil (30 min incubation)), 30-90 units/mg protein (using triacetin)

C3389

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アセチルコリンエステラーゼ Electrophorus electricus（電気ウナギ）由来, Type VI-S, lyophilized powder, 200-1,000 units/mg protein

P3627

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酸性ホスファターゼコムギ胚芽由来, ≥0.4 unit/mg solid

L1254

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L-乳酸デヒドロゲナーゼウサギ筋肉由来, Type XI, lyophilized powder, 600-1,200 units/mg protein

A7011

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アルコールデヒドロゲナーゼ from Saccharomyces cerevisiae, ≥300 units/mg protein, lyophilized powder (contains buffer salts), M_w 141-151 kDa

L4777

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リパーゼ・アクリル樹脂 from Candida antarctica, ≥5,000 U/g, recombinant, expressed in Aspergillus niger

C3755

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クレアチンホスホキナーゼウサギ筋肉由来, Type I, salt-free, lyophilized powder, ≥150 units/mg protein

G5160

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β-ガラクトシダーゼ from Aspergillus oryzae, ≥8.0 units/mg solid

D1806

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Taq DNAポリメラーゼ Thermus aquaticus由来, with 10× PCR reaction buffer containing MgCl₂

P3752

Sigma-Aldrich

酸性ホスファターゼジャガイモ由来, lyophilized powder, ≥0.5 unit/mg solid

D4545

Sigma-Aldrich

Taq DNAポリメラーゼ Thermus aquaticus由来, with 10× PCR reaction buffer without MgCl₂

G5635

Sigma-Aldrich

β-ガラクトシダーゼ 大腸菌由来, Grade VIII, lyophilized powder, ≥500 units/mg protein

A3263

Sigma-Aldrich

アルコールデヒドロゲナーゼ from Saccharomyces cerevisiae, powder, ≥300 units/mg protein, mol wt ~141,000 (four subunits)

L2500

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L-乳酸デヒドロゲナーゼウサギ筋肉由来, Type II, ammonium sulfate suspension, 800-1,200 units/mg protein

P9136

Sigma-Aldrich

ピルビン酸キナーゼウサギ筋肉由来, Type III, lyophilized powder, 350-600 units/mg protein

H4502

Sigma-Aldrich

ヘキソキナーゼ from Saccharomyces cerevisiae, Type F-300, lyophilized powder, ≥130 units/mg protein (biuret)

G2267

Sigma-Aldrich

グリセルアルデヒド 3-リン酸デヒドロゲナーゼウサギ筋肉由来, lyophilized powder, ≥75 units/mg protein

L0382

Sigma-Aldrich

リパーゼブタ膵臓由来, Type VI-S, ≥20,000 units/mg protein, lyophilized powder

62288

Sigma-Aldrich

リパーゼB, Candida antarctica由来, 組換え体 from Aspergillus oryzae, powder, beige, ~9 U/mg

D5540

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ジアホラーゼ from Clostridium kluyveri, lyophilized powder, 3.0-20.0 units/mg protein (biuret)

A3809

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アスパラギナーゼ 大腸菌由来, lyophilized powder, 100-300 units/mg protein (biuret)

P1506

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ピルビン酸キナーゼウサギ筋肉由来, Type II, ammonium sulfate suspension, 350-600 units/mg protein

M7441

Sigma-Aldrich

モノアミンオキシダーゼB ヒト, recombinant, expressed in baculovirus infected BTI insect cells

C2888

Sigma-Aldrich

アセチルコリンエステラーゼ Electrophorus electricus（電気ウナギ）由来, Type V-S, lyophilized powder, ≥1,000 units/mg protein

A8656

Sigma-Aldrich

アルコールデヒドロゲナーゼ from Saccharomyces cerevisiae

G2751

Sigma-Aldrich

グルタミン酸-オキサロ酢酸トランスアミナーゼブタ心臓由来, Type I, ammonium sulfate suspension, 200-500 units/mg protein

P9279

Sigma-Aldrich

ホスホリパーゼA₂ from honey bee venom (Apis mellifera), salt-free, lyophilized powder, 600-2400 units/mg protein

L2625

Sigma-Aldrich

L-乳酸デヒドロゲナーゼウシ心臓由来, Type III, ammonium sulfate suspension, ≥500 units/mg protein

62301

Sigma-Aldrich

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