796-0657

HPLC Analysis of Proteins on Discovery^® BIO PolyMA-WAX (Analyte Set #1)

application for HPLC

Propriedades

• descrição: Separated on Discovery^® BIO PolyMA-WAX
• técnica(s): HPLC: suitable
• parâmetros de teste: column: Discovery BIO PolyMA-WAX, 5 cm x 4.6 mm, 5 μm particles (59602-U); mobile phase: [A] 10 mM Tris, pH 8.0 with HCl; [B] 10 mM Tris, 0.5 M NaCl, pH 8.0 with HCl; 5 to 100% B in 15 min. (linear); flow rate: 0.5 mL/min; detector: UV, 280 nm; injection: 10 μg trypsin inhibitor, 5 μg conalbumin, myoglobin or 10 μg ribonuclease A, 5 μg cytochrome c, a-chymotrypsinogen A
• adequação: application for HPLC
• aplicação(ões): life science and biopharma

Descrição

Nota de análise

The large pore diameter of Discovery BIO PolyMA-SCX and PolyMA-WAX particles allows full access to small peptides and very large proteins and protein aggregates. This Figure shows the separation of proteins that vary from 18 to 80 kDA.

Informações legais

Discovery is a registered trademark of Merck KGaA, Darmstadt, Germany

Analito

Trypsin Inhibitor