T2327

Trypsin inhibitor

lyophilized powder, ≥95% (Kunitz inhibitor, SDS-PAGE)

• Sinônimo(s): Kunitz Inhibitor
• Número CAS: 9035-81-8
• Número CE: 232-906-9
• Número MDL: MFCD00082102
• Código UNSPSC: 12352204
• NACRES: NA.77

Propriedades

• Nome do produto: Trypsin Inhibitor from Glycine max (soybean), BioUltra, lyophilized powder, ≥95% (Kunitz inhibitor, SDS-PAGE)
• fonte biológica: Glycine max (soybean)
• Nível de qualidade: 200
• linha de produto: BioUltra
• Ensaio: ≥95% (Kunitz inhibitor, SDS-PAGE)
• Formulário: lyophilized powder
• temperatura de armazenamento: 2-8°C

Descrição

Descrição geral

Trypsin Inhibitor from Glycine max (soybean) also known as Kunitz trypsin inhibitor is a 21 kDa protein with a single trypsin binding reactive site.

Aplicação

Trypsin Inhibitor from Glycine max (soybean) has been used:

• as a standard protein to measure the amount of endogenous trypsin inhibitor present in midgut lysate (M1) of Riptortus pedestris
• as a standard to compare the trypsin inhibitory activity of the purified protein
• to monitor the trypsin inhibitory activity by fractionating in MonoS cation exchange chromatography
• as an trypsin inhibitor

Ações bioquímicas/fisiológicas

Soybean trypsin inhibitor inhibits trypsin and to a lesser extent chymotrypsin and plasmin. It forms a 1:1 stoichiometric complex with trypsin. Upon formation of this complex, trypsin may cleave a single arginine-isoleucine bond in the inhibitor. Dissociation of this complex may yield the modified form or the native inhibitor. At the optimal pH for trypsin binding (pH 8.0), the association constant is ≥ 10x10⁸.

Trypsin Inhibitor from Glycine max (soybean) binds with the active site of trypsin enzyme, in a competitive inhibition manner.

Definição da unidade

One trypsin unit will produce a ΔA₂₅₃ of 0.001 per min with BAEE as substrate at pH 7.6 at 25 °C; reaction volume 3.2 ml, 1 cm light path.

Nota de preparo

Further purification of T9128 yielding an electrophoretically pure Kunitz inhibitor with increased activity.

Trypsin inhibitor is soluble in water and phosphate buffers at concentrations of 10 mg/ml or higher. Solutions at higher concentrations may be hazy and have a yellow to amber color.

Nota de análise

One mg will inhibit ≥1.0 mg of trypsin with activity of approx. 10,000 BAEE units per mg protein.

Outras notas

View more information on Trypsin Inhibitor.

Informações de segurança

• Pictogramas: GHS08
• Palavra indicadora: Danger
• Frases de perigo: H317,H334
• Declarações de precaução: P261 - P272 - P280 - P284 - P302 + P352 - P333 + P313
• Classificações de perigo: Resp. Sens. 1 - Skin Sens. 1
• Código de classe de armazenamento: 11 - Combustible Solids
• Classe de risco de água (WGK): WGK 3
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable
• Equipamento de proteção individual: Eyeshields, Gloves, type N95 (US)