T6414

Trypsin inhibitor from Glycine max (soybean)

solution, sterile-filtered, suitable for, suitable for cell culture

• Sinônimo(s): SBTI
• Número CAS: 9035-81-8
• Número MDL: MFCD00082103
• Código UNSPSC: 12352204
• NACRES: NA.75

Propriedades

• fonte biológica: Glycine max (soybean)
• Nível de qualidade: 100
• esterilidade: sterile-filtered
• linha de produto: BioReagent
• Formulário: solution
• peso molecular: 20,100 Da
• concentração: 10 mg/mL
• técnica(s): cell culture | mammalian: suitable
• solubilidade: balanced salt solution: 1 mg/mL; concentrate: >10 mg/mL, hazy, amber-yellow; phosphate buffer: 10 mg/mL; water: 10 mg/mL; serum-free medium: soluble
• adequação: suitable for
• nº de adesão UniProt: P01070
• Condições de expedição: dry ice
• temperatura de armazenamento: −20°C
• Informações sobre genes: Glycine max (soybean) ... KTI3(547831)

Descrição

Aplicação

Optimized for passage of endothelial cell cultures.
Trypsin inhibitor from Glycine max (soybean) was used for the following applications:

• Filteration and seeding of dispersed acini
• Relative quantification of phospho-tau and immunoblotting analysis
• Subcloning and transformation of VIL-4 and IL-4 cDNA, and protein expression and purification (trypsin inhibitor form glycine max is used as positive control).

Ações bioquímicas/fisiológicas

This inhibitor acts against trypsin, and chymotrypsin and plasmin to a lesser extent. It will also inhibit proteases with mechanisms similar to trypsin, plasma kallikrein and coagulation Factor X. The trypsin inhibitor will not act against metalloproteases, tissue-baseed kallikrein, acid proteases, or thio proteases. This inhibitor acts by forming a 1:1 stoichiometric complex with the protease active site, and then cleaving a single arginine-isoleucine bond on the inhibitor. The inhibition is both reversible and pH dependent.

Outras notas

Contains chromatographically purified soybean trypsin inhibitor and iron supplemented calf serum.

View more information on Trypsin Inhibitor.

Definição da unidade

One trypsin unit = A₂₅₃ of 0.001 per minute with N-alpha-benzoyl-L-arginine ethyl ester (BAEE) as substrate at pH 7.6 at 25 °C.

Nota de preparo

Prepared in Dulbecco′s Phosphate Buffered Saline without calcium and magnesium.

The trypsin inhibitor is soluble in water and phosphate buffers at 10 mg/mL. It is soluble in balanced salt solutions at 1 mg/mL and in serum-free media. Concentrated solutions greater than 10 mg/mL may be hazy and have a yellow to amber color. After trypsinizing cells, resuspend in 1 mL trypsin inhibitor solution at 1 mg/mL for every mL of trypsin solution used for dissociation. The cell suspension should then be centrifuged at 1000 rpm, forming a cell pellet.

Solutions can retain activity when stored short-term at 2-8° C. Solutions are stable in frozen aliquots at -20°C.

Informações de segurança

• Pictogramas: GHS08
• Palavra indicadora: Danger
• Frases de perigo: H317,H334
• Declarações de precaução: P261 - P272 - P280 - P284 - P302 + P352 - P333 + P313
• Classificações de perigo: Resp. Sens. 1 - Skin Sens. 1
• Código de classe de armazenamento: 12 - Non Combustible Liquids
• Classe de risco de água (WGK): WGK 2
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable
• Equipamento de proteção individual: Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter