T9378

Trypsin inhibitor

crude powder

• Sinônimo(s): Trypsin Inhibitor from lima beans
• Número CAS: 9035-81-8
• Número CE: 232-906-9
• Número MDL: MFCD00082102
• Código UNSPSC: 12352202
• NACRES: NA.77

Propriedades

• product name: Trypsin inhibitor from Phaseolus limensis (lima bean), Type II-L, crude powder
• fonte biológica: Phaseolus limensis (lima bean)
• tipo: Type II-L
• forma: crude powder
• peso molecular: 9 kDa
• solubilidade: 0.067 M sodium phosphate buffer, pH 7.6: 1 mg/mL
• temperatura de armazenamento: 2-8°C

Descrição

Aplicação

Trypsin inhibitor from Phaseolus limensis (lima bean) has been used:

• in the inhibition of trypsin in human plasma
• in the termination of proteolytic digestion in rabbit skeletal muscles
• in the inhibition of salivary glands enzyme extract from Lygus lineolaris
• in the inhibition of proteolytic activity in Agave tequilana enzyme extract

Trypsin inhibitor has been used as an affinity ligand for isolation of elastase-type enzymes.

Ações bioquímicas/fisiológicas

Trypsin inhibitor from lima beans belongs to Bowman-Birk family of protease inhibitors. It is 9 kDa in molecular weight and contains nine disulfide bridges and is highly thermostable. It has trypsin and chymotrypsin binding subdomains.

propriedades físicas

Monomer has an apparent molecular weight of approx. 9,000 Da but undergoes a concentration and pH-dependent dimerization.

Definição da unidade

One trypsin unit will produce a ΔA₂₅₃ of 0.001 per min with BAEE as substrate at pH 7.6 at 25 °C; reaction volume 3.2 mL, 1 cm light path.

Nota de preparo

Prepared by a modification of the method of Tauber, H., et al., J. Biol. Chem., 179, 1155 (1949) (modified).

Nota de análise

One mg will inhibit ≥0.8 mg of trypsin with activity of approx. 10,000 BAEE units per mg protein.

Outras notas

View more information on Trypsin Inhibitor.

Informações de segurança

• Pictogramas: GHS08
• Palavra indicadora: Danger
• Frases de perigo: H317,H334
• Declarações de precaução: P261 - P272 - P280 - P284 - P302 + P352 - P333 + P313
• Classificações de perigo: Resp. Sens. 1 - Skin Sens. 1
• Código de classe de armazenamento: 11 - Combustible Solids
• Classe de risco de água (WGK): WGK 3
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable
• Equipamento de proteção individual: Eyeshields, Gloves, type N95 (US)