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Key Documents

T6763

Sigma-Aldrich

Trypsin Acetylated from bovine pancreas

Type V-S, ≥8,500 BAEE units/mg protein (biuret)

Sinônimo(s):

Acetyltrypsin

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About This Item

Número da licença da enzima:
Número MDL:
Código UNSPSC:
12352204
NACRES:
NA.54

fonte biológica

bovine pancreas

Nível de qualidade

tipo

Type V-S

forma

powder

atividade específica

≥8,500 BAEE units/mg protein (biuret)

nº de adesão UniProt

temperatura de armazenamento

−20°C

Informações sobre genes

Descrição geral

The trypsin molecule has two domains: one is related to the enzyme active site and the tryptophan residues; the other is related to the 8-anilinonaphthalene-1-sulfonate binding.

Aplicação

Trypsin has been used in a study to assess the ontogeny of the endocrine pancreas in the fetal/newborn baboon. Trypsin digestion and hydrogen/deuterium exchange support the transition between inward- and outward-facing conformations during the catalytic cycle of the bacterial multidrug ATP-binding cassette transporter.

Ações bioquímicas/fisiológicas

Lys-acetylated trypsin is less susceptible to autolysis than the native form.

Definição da unidade

One BAEE unit will produce a ΔA253 of 0.001 per min at pH 7.6 at 25° C using BAEE as substrate. Reaction volume = 3.2 mL (1 cm light path).

Nota de preparo

Prepared by the method of Labouesse, J. and Gervais, M., Eur. J. Biochem., 2, 215 (1967).

Outras notas

View more information on trypsin at www.sigma-aldrich.com/enzymeexplorer.

Pictogramas

Health hazardExclamation mark

Palavra indicadora

Danger

Frases de perigo

Classificações de perigo

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

Órgãos-alvo

Respiratory system

Código de classe de armazenamento

11 - Combustible Solids

Classe de risco de água (WGK)

WGK 3

Equipamento de proteção individual

dust mask type N95 (US), Eyeshields, Faceshields, Gloves


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