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RTRYP-RO

Roche

Trypsin recombinant, Proteomics Grade

recombinant enzyme expressed in Pichia pastoris

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About This Item

Número da licença da enzima:
3.4.21.4 (BRENDA, IUBMB)
Código UNSPSC:
12352204
NACRES:
NA.21

recombinante

expressed in Pichia pastoris

Nível de qualidade

100

Ensaio

95% (gel filtration)

forma

lyophilized

atividade específica

≥150 units/mg protein

embalagem

pkg of 4 × 100 μg (03708969001)
pkg of 4 × 25 μg (03708985001)

fabricante/nome comercial

Roche

temperatura de armazenamento

−20°C

Categorias relacionadas

Descrição geral

This trypsin preparation is generated from the recombinant Pichia pastoris strain and is, thus, free of chymotrypsin. Trypsin recombinant, Proteomics Grade, exhibits high specific activity.

Especificidade

Serine endopeptidase, hydrolyzing specifically proteins and peptides at the carboxy side of the basic amino acids Arg and Lys. Amide and ester bonds of Arg and Lys are also cleaved.

Aplicação

Trypsin recombinant, Proteomics Grade, is used to specifically digest proteins separated by 2D gel electrophoresis or liquid chromatographic methods during sample preparation for mass spectrometry.

Nota de preparo

Stabilizers: Ca2+ in mM concentration range
Inhibitors: TLCK, DFP, PMSF, leupeptin, soybean trypsin inhibitor, trypsin inhibitor from hen egg, aprotinin, α2-macroglobulin, α1-antitrypsin, APMSF, and antipain.
Storage conditions (working solution): -15 to -25°C
The reconstituted solution is stable for 1 month at -15 to -25°C. Avoid repeated freezing/thawing!

Reconstituição

Following reconstitution in 10 mM HCl, Trypsin, recombinant, proteomics grade is stable at -15 to -25 °C for up to one month. After first reconstitution the product must be stored in appropriate aliquots to avoid repeated freezing and thawing.

Outras notas

For life science research only. Not for use in diagnostic procedures.

Pictogramas

Exclamation markHealth hazard
GHS07,GHS08

Palavra indicadora

Danger

Classificações de perigo

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

Órgãos-alvo

Respiratory system

Código de classe de armazenamento

11 - Combustible Solids

Classe de risco de água (WGK)

WGK 1

Ponto de fulgor (°F)

does not flash

Ponto de fulgor (°C)

does not flash

Certificados de análise (COA)

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A Acera et al.
Eye (London, England), 25(9), 1225-1233 (2011-06-28)
To analyze tear protein profile variations in patients with keratoconus (KC) and to compare them with those of control subjects. Tears from 12 normal subjects and 12 patients with KC were analyzed by two-dimensional gel electrophoresis (2-DE) and liquid chromatography-mass
Alexander Schmidt et al.
Methods in molecular biology (Clifton, N.J.), 492, 21-38 (2009-02-26)
Proteomics may be defined as the systematic analysis of proteins expressed in a given organism (Electrophoresis 16:1090-1094, 1995). Important technical innovations in mass spectrometry (MS), protein identification methods, and database annotation, over the past decade, now make it possible to

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