TRYPSEQM-RO
Roche
Trypsin Sequencing Grade, modified
from bovine pancreas
Sinônimo(s):
Trypsin
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About This Item
Produtos recomendados
fonte biológica
bovine pancreas
Nível de qualidade
Formulário
lyophilized (salt-free)
peso molecular
24.000 g/mol
embalagem
pkg of 4 × 100 μg (11418033001)
pkg of 4 × 25 μg (11418025001)
fabricante/nome comercial
Roche
condição de armazenamento
(Keep container tightly closed in a dry and well-ventilated place.)
concentração
0.01-0.2 % (w/w)
técnica(s)
protein sequencing: suitable
Impurezas
Chymotrypsin
cor
white
pH ideal
8.0
solubilidade
10 g/L
adequação
suitable for protein modification
nº de adesão UniProt
aplicação(ões)
life science and biopharma
atividade externa
Contaminating activities corresponds
Chymotrypsin , contains
temperatura de armazenamento
2-8°C
Informações sobre genes
cow ... PRSS1(780933)
Descrição geral
Trypsin Sequencing Grade, modified, is isolated from bovine pancreas as a highly purified and specific protease, and subsequently modified.
Trypsin is a highly efficient and specific protease widely used in proteomics for protein digestion. It produces short peptides with specific characteristics that are compatible with current separation and identification methods such as liquid chromatography, mass spectrometry (MS).
Inhibitors: TLCK, DFP, PMSF, leupeptin, soybean trypsin inhibitor, trypsin inhibitor from hen egg, aprotinin, α2-macroglobulin,α1-antitrypsin, APMSF, and antipain.
Trypsin is a highly efficient and specific protease widely used in proteomics for protein digestion. It produces short peptides with specific characteristics that are compatible with current separation and identification methods such as liquid chromatography, mass spectrometry (MS).
Inhibitors: TLCK, DFP, PMSF, leupeptin, soybean trypsin inhibitor, trypsin inhibitor from hen egg, aprotinin, α2-macroglobulin,α1-antitrypsin, APMSF, and antipain.
Especificidade
Trypsin is a serine endopeptidase. At pH 7.5–9, it specifically hydrolyzes proteins and peptide bonds C-terminally of Iysine and arginine. Amide and ester bonds of Arg and Lys are also cleaved. The specificity of Trypsin Sequencing Grade, modified, is verified with the oxidized B-chain of insulin (insulin Box) as a substrate. High concentrations of Trypsin Sequencing Grade, modified, one part by weight enzyme with 9 parts by weight insulin Box, are incubated for 18 hours to detect traces ofchymotrypsin impurities.
Aplicação
Use Trypsin Sequencing Grade, modified, to generate glycopeptides from purified glycoproteins.
It is used for:
It is used for:
- Protein-structure elucidation
- Tryptic mapping
- Fingerprinting analysis
- Sequence analysis
- Translocation studies
- Protein identification
- Protein digestion during lipoprotein preparation for liquid chromatography-tandem mass spectrometry (LC-MS/MS)
Qualidade
Purity: Free of impurities that may interfere with the separation of peptides in reversed-phase HPLC.
Nota de preparo
Working concentration: 1/100 to 1/5 of the protein by weight
Storage conditions (working solution): -15 to -25 °C
Trypsin Sequencing Grade, modified, is more resistant to autolysis, even at pH values in the neutral and weakly basic range. The enzyme can be used in high concentrations.
A solution in 1% acetic acid or 1 mM HCI can be used for up to one week when stored at 2 to 8° C. Stored in aliquots at -15 to -25 °C, the solution is stable for at least one year without loss of activity.
Storage conditions (working solution): -15 to -25 °C
Trypsin Sequencing Grade, modified, is more resistant to autolysis, even at pH values in the neutral and weakly basic range. The enzyme can be used in high concentrations.
A solution in 1% acetic acid or 1 mM HCI can be used for up to one week when stored at 2 to 8° C. Stored in aliquots at -15 to -25 °C, the solution is stable for at least one year without loss of activity.
Armazenamento e estabilidade
Store dry
Outras notas
For life science research only. Not for use in diagnostic procedures.
Palavra indicadora
Danger
Frases de perigo
Declarações de precaução
Classificações de perigo
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
Órgãos-alvo
Respiratory system
Código de classe de armazenamento
11 - Combustible Solids
Classe de risco de água (WGK)
WGK 1
Ponto de fulgor (°F)
Not applicable
Ponto de fulgor (°C)
Not applicable
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