EMS0006

Recombinant Trypsin

Proteomics Grade, lyophilized powder, recombinant, expressed in Pichia pastoris

• Sinônimo(s): Mass Spectrometry Trypsin, Proteomics grade Trypsin, rTrypsin
• Código UNSPSC: 12352204
• NACRES: NA.26

Propriedades

• recombinante: expressed in Pichia pastoris
• Nível de qualidade: 200
• grau: Proteomics Grade
• Formulário: lyophilized powder
• atividade específica: ≥10,000 units/mg protein
• adequação: suitable for ; suitable for mass spectrometry
• nº de adesão UniProt: P00761
• Condições de expedição: wet ice
• temperatura de armazenamento: 2-8°C

Descrição

Descrição geral

Trypsin is a major proteolytic enzyme, synthesized as a preproenzyme by pancreas and is stored as proenzyme trypsinogen in secretory granules. Trypsin belongs to the family of serine proteases that are characterized by the catalytic triad His57, Asp102 and Ser195. Trypsin is routinely used in proteomics research for peptide mapping and protein sequence work due to its highly specific cleavage resulting in a limited number of tryptic peptides. Trypsin is a pancreatic serine endoprotease which hydrolyzes peptide bonds specifically at the carboxyl side of arginine and lysine residues. The rate of hydrolysis is slower if an acidic residue is on either side of the cleavage site and cleavage may not occur if a proline residue is on the carboxyl side. The enzyme also exhibits esterase and amidase activities. Trypsin has an average molecular mass of 23.29 kDa and a pH optimum near 8.0. This product is prepared from recombinant trypsin, porcine sequence. It is naturally devoid of chymotryptic activity. This high-quality trypsin is suitable for proteomics use.

Ações bioquímicas/fisiológicas

Trypsin plays an important role in the digestion of consumed protein and contributes to the activation of other proteolytic enzymes like chemotrypsin and elastase.

Informações de segurança

• Pictogramas: GHS08,GHS05,GHS07,GHS09
• Palavra indicadora: Danger
• Frases de perigo: H290,H314,H317,H334,H335,H411
• Declarações de precaução: P260 - P273 - P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338
• Classificações de perigo: Aquatic Chronic 2 - Eye Dam. 1 - Met. Corr. 1 - Resp. Sens. 1 - Skin Corr. 1A - Skin Sens. 1 - STOT SE 3
• Órgãos-alvo: Respiratory system
• Perigos de suplementos: EUH071
• Código de classe de armazenamento: 8A - Combustible corrosive hazardous materials
• Classe de risco de água (WGK): WGK 3
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable