11418467001

Chymotrypsin Sequencing Grade

from bovine pancreas

• Sinônimo(s): chymotrypsin, protease
• Número da licença da enzima: 3.4.21.1 (BRENDA, IUBMB)
• Código UNSPSC: 12352204

Propriedades

• fonte biológica: bovine pancreas
• Nível de qualidade: 100
• Ensaio: ≥90%
• forma: lyophilized (salt-free)
• atividade específica: ≥70 units/mg protein (at 25 °C, with ATEE as a substrate)
• peso molecular: M_r 25 kDa
• embalagem: pkg of 4 × 25 μg
• fabricante/nome comercial: Roche
• técnica(s): protein sequencing: suitable
• pH ideal: 7.0-9.0
• Condições de expedição: wet ice
• temperatura de armazenamento: 2-8°C

Descrição

Descrição geral

Chymotrypsin Sequencing Grade is isolated as a specific protease in pure form from bovine pancreas.

Especificidade

Serine endopeptidase that specifically hydrolyzes peptide bonds at the C-terminial of Tyr, Phe, and Trp. Leu, Met, Ala, Asp, and Glu are cleaved at a lower rate. Acts also upon amides and esters of susceptible amino acids. The specificity of Chymotrypsin Sequencing Grade is tested with melittin as a substrate.

Aplicação

Use Chymotrypsin Sequencing Grade for the hydrolysis of proteins by chymotrypsin alone or in combination with other proteases. It is suitable for peptide mapping, fingerprinting, and sequence analysis.

Qualidade

Purity: Free of impurities that may interfere with the specific cleavage or separation of peptides in reversed-phase HPLC.

Nota de preparo

Working concentration: The recommended amount of enzyme is 1/200 to 1/20 of the quantity of protein by weight.
Storage conditions (working solution): 2 to 8 °C
A solution in 1 mM HCl can be stored for up to one week at 2 to 8 °C.
Inhibitors: Aprotinin, DFP, PMSF, phenothiazine-N-carbonyl chloride, TPCK, ZPCK, α2-macroglobulin, α1-antitrypsin, soybean trypsin inhibitor, and chymostatin. No inhibition by APMSF.

Reconstituição

Dissolve lyophilized chymotrypsin sequencing grade in 1mM HCl. The proteins to be sequenced are dissolved in digestion buffer (100mM Tris-HCl, 10mM CaCl₂, pH 7.8).

Outras notas

For life science research only. Not for use in diagnostic procedures.

Informações de segurança

• Pictogramas: GHS07,GHS08
• Palavra indicadora: Danger
• Frases de perigo: H315,H319,H334,H335
• Declarações de precaução: P261 - P264 - P280 - P284 - P304 + P340 + P312 - P342 + P311
• Classificações de perigo: Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
• Órgãos-alvo: Respiratory system
• Código de classe de armazenamento: 11 - Combustible Solids
• Classe de risco de água (WGK): WGK 1
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable