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Key Documents

10108057001

Roche

Pepsin

lyophilized (salt-free), ~2500 units/mg protein (At 37 °C with hemoglobin as the substrate. One unit is the enzyme activity which liberates the amount of Tyr producing an increase in the absorbance of 0.001/minute at 280 nm.)

Sinônimo(s):

pepsin

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About This Item

Número da licença da enzima:
Código UNSPSC:
12352204

fonte biológica

pig gastric mucosa

Nível de qualidade

forma

lyophilized (salt-free)

atividade específica

~2500 units/mg protein

embalagem

pkg of 1 g

fabricante/nome comercial

Roche

pH ideal

1.8-2.2

Condições de expedição

wet ice

temperatura de armazenamento

2-8°C

Descrição geral

Pepsin is an aspartic endoproteinase isolated from pig gastric mucosa. It is mainly used for the unspecific hydrolysis of proteins and peptides in acidic media. It also provides a limited hydrolysis of native immunoglobulins to yield biologically active fragments.

Especificidade

Aspartic endopeptidase with relatively broad specificity. Preferential cleavage: hydrophobic, preferably aromatic, residues in P1 and P1′ positions.

Aplicação

Pepsin has been used for the digestion of liver tissue obtained from rat.

Nota de preparo

Working concentration: 0.05 to 0.5 mg/ml
Storage conditions (working solution): A pepsin solution is stable at 2 to 8 °C, at least for one week, at neutral pH and under germfree conditions. Frozen aliquots of the enzyme solution are expected to be more stable.

A stock solution in 10 mM HCl (5-10 mg/ml) should be stable for at least one week at 2 to 8 °C, too. Storage light protected, tightly closed.

A short (2 minutes) incubation at 40 °C has negligible effect on the activity of lyophilized pepsin or pepsin in solution.

Nota de análise

At 37 °C with hemoglobin as the substrate. One unit is the enzyme activity which liberates the amount of Tyr producing an increase in the absorbance of 0.001/minute at 280 nm.

Outras notas

For life science research only. Not for use in diagnostic procedures.

Pictogramas

Exclamation markHealth hazard

Palavra indicadora

Danger

Frases de perigo

Classificações de perigo

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

Órgãos-alvo

Respiratory system

Código de classe de armazenamento

11 - Combustible Solids

Classe de risco de água (WGK)

WGK 1

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable


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