P7125
Pepsina
powder, ≥400 units/mg protein
Sinônimo(s):
Pepsina, Pepsina A
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About This Item
Produtos recomendados
fonte biológica
Porcine gastric mucosa
Nível de qualidade
forma
powder
atividade específica
≥400 units/mg protein
peso molecular
35 kDa
solubilidade
10 mM HCl: soluble 1.0 mg/mL, clear to faintly turbid, colorless
nº de adesão UniProt
temperatura de armazenamento
2-8°C
Informações sobre genes
pig ... LOC396892(396892)
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Categorias relacionadas
Aplicação
Pepsin is a peptidase used to digest proteins and is commonly used in the preparation of Fab fragments from antibodies. Pepsin, from porcine gastric mucosa, has been used to hydrolyze dry cervical samples in mice. Product P7125 is provided in powder form. Product P7125 has been used to denature DNA from kidney cells and to digest pathology samples from anal canal carcinomas (ACC) biopsies prior to EGFR staining.
The enzyme from Sigma has been used to obtain total vitamin B12 content in food products prior using immunoaffinity columns It has also been used to digest minced soft tissue of snails prior to the isolation of the third stage larvae (L3).
A clivagem da pepsina pode ser usada para produzir fragmentos de anticorpos F(ab′)2. pepsina na página www.sigma-aldrich.com/enzymeexplorer.
Ações bioquímicas/fisiológicas
It does not cleave at valine, alanine, or glycine linkages. Z-L-tyrosyl-L-phenylalanine, Z-L-glutamyl-L-tyrosine, or Z-L-methionyl-L-tyrosine may be used as substrates for pepsin digestion. Pepsin is inhibited by several phenylalanine-containing peptides.
Pepsin hydrolyzes peptide bonds, not amide or ester linkages. Pepsin cleaves peptides with an aromatic acid on either side of the peptide bond. Sulfur-containing amino acids increase susceptibility to hydrolysis when they are close to the peptide bond. Pepsin preferentially cleaves at the carboxyl side of phenylalanine and leucine and at the carboxyl side of glutamic acid residues. Cleaves Phe-Val, Gln-His, Glu-Ala, Ala-Leu, Leu-Tyr, Tyr-Leu, Gly-Phe, Phe-Phe and Phe-Tyr bonds in the β chain of insulin
Pepsin is the major proteolytic enzyme produced in the stomach. It digests proteins through the cleavage of interior peptide linkages.
Pepsin is the major proteolytic enzyme produced in the stomach. It digests proteins through the cleavage of interior peptide linkages.
Clivagem preferencial: resíduos hidrofóbicos e aromáticos nas posições P1 e P1′. Cliva ligações Fen-Val, Gln-His, Glu-Ala, Ala-Leu, Leu-Tir, Tir-Leu, Gli-Fen, Fen-Fen e Fen-Tir na cadeia β da insulina
Definição da unidade
One unit will produce a ΔA280 of 0.001 per min at pH 2.0 at 37 °C, measured as TCA-soluble products using hemoglobin as substrate. (Final volume = 16 mL. Light path = 1 cm.)
Nota de análise
O pH ideal é 2-4. Ativa em 4 M de ureia e 3 M de guanidina-HCl. Estável a 60 ºC. A pepsina é inativada de modo irreversível em pH 8,0-8,5.
Protein determined by E1%/280
Outras notas
View more information on pepsin at www.sigma-aldrich.com/enzymeexplorer.
inibidor
Nº do produto
Descrição
Preços
Palavra indicadora
Danger
Frases de perigo
Declarações de precaução
Classificações de perigo
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
Órgãos-alvo
Respiratory system
Código de classe de armazenamento
11 - Combustible Solids
Classe de risco de água (WGK)
WGK 1
Ponto de fulgor (°F)
Not applicable
Ponto de fulgor (°C)
Not applicable
Equipamento de proteção individual
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
Certificados de análise (COA)
Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.
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