M2414
Meio mínimo essencial de Eagle
With Earle′s salts and reduced NaHCO₃. without ʟ-glutamine, liquid, sterile-filtered, suitable for cell culture
Sinônimo(s):
MEM
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About This Item
Código UNSPSC:
12352207
NACRES:
NA.75
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Nome do produto
Meio mínimo essencial de Eagle, Modified, with Earle′s salts and reduced NaHCO3. without L-glutamine, liquid, sterile-filtered, suitable for cell culture
Nível de qualidade
esterilidade
sterile-filtered
Formulário
liquid
técnica(s)
cell culture | mammalian: suitable
Impurezas
endotoxin, tested
componentes
phenol red: 0.011 g/L
NaHCO3: 0.85 g/L
glucose: 1.0 g/L
L-glutamine: no
Condições de expedição
ambient
temperatura de armazenamento
2-8°C
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Descrição geral
Minimum Essential Medium (MEM), developed by Harry Eagle, is one of the most widely used of all synthetic cell culture media. Early attempts to cultivate normal mammalian fibroblasts and certain subtypes of HeLa cells revealed they had specific nutritional requirements that could not be met by Eagle′s Basal Medium (BME). Subsequent studies using these and other cells in culture indicated additions to BME could be made to aid growth of a wider variety of fastidious cells.
MEM, which incorporates these modifications, includes higher concentrations of amino acids so the medium more closely approximates the protein composition of cultured mammalian cells. MEM has been used for cultivation of a wide variety of cells grown in monolayers. Optional supplementation of non-essential amino acids to the formulations that incorporate either Hanks′ or Earle′s salts has broadened the usefulness of this medium.
MEM, which incorporates these modifications, includes higher concentrations of amino acids so the medium more closely approximates the protein composition of cultured mammalian cells. MEM has been used for cultivation of a wide variety of cells grown in monolayers. Optional supplementation of non-essential amino acids to the formulations that incorporate either Hanks′ or Earle′s salts has broadened the usefulness of this medium.
Aplicação
Recommended for use in a carbon dioxide-free culture system.
Outras notas
This product lacks L-Ala; L-Asn; L-Glu; Gly; L-Pro; L-Ser and L-Gln.
May also be supplemented with HEPES to raise the buffering range.
Reconstituição
Supplement with 0.292 g/L L-glutamine.
geralmente comprado junto com este produto
Nº do produto
Descrição
Preços
Código de classe de armazenamento
12 - Non Combustible Liquids
Classe de risco de água (WGK)
WGK 1
Ponto de fulgor (°F)
Not applicable
Ponto de fulgor (°C)
Not applicable
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