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Key Documents

M7145

Sigma-Aldrich

MEM Non-essential Amino Acid Solution (100×)

Without ʟ-glutamine, liquid, sterile-filtered, suitable for cell culture

Sinônimo(s):

MEM NEAA

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About This Item

Código UNSPSC:
12352207
eCl@ss:
32160406
NACRES:
NA.75

product name

MEM Non-essential Amino Acid Solution (100×), without L-glutamine, liquid, sterile-filtered, BioReagent, suitable for cell culture

Nível de qualidade

esterilidade

sterile-filtered

linha de produto

BioReagent

forma

liquid

técnica(s)

cell culture | mammalian: suitable

Impurezas

endotoxin, tested

temperatura de armazenamento

2-8°C

Descrição geral

Eagle′s minimum essential medium (MEM) is a synthetic culture medium, which is composed of balanced salt solutions, and amino acids and vitamins. These components are essential for the growth of cultured cells. MEM might be supplemented with non-essential amino acids, to make MEM non-essential amino acid solution. The non-essential amino acids include L-alanine, L-asparagine, L-aspartic acid, L-glycine, L-serine, L-proline and L-glutamic acid.

Aplicação

Adding supplements of amino acids to media both stimulates growth and prolongs the viability of cells in culture. MEM Non-essential Amino Acid Solution supplement adds nutrients and reduces the biosynthetic burden on cell in vitro.
MEM Non-essential Amino Acid Solution has been used in Dulbecco′s minimum essential media for the maintenance of mammalian cells. It has been supplemented in medium prepared for oocyte maturation.

Ações bioquímicas/fisiológicas

Studies on hybridoma culture shows that addition of Eagle′s minimum essential medium (MEM) non-essential amino acid leads to enhanced membrane stability, during death phase of the culture. This also results in increased antibody secretion during death phase, and decreased lactate concentration.

Código de classe de armazenamento

10 - Combustible liquids

Classe de risco de água (WGK)

WGK 3

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable


Certificados de análise (COA)

Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.

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Excessive concentration of glucose during in vitro maturation impairs the developmental competence of bovine oocytes after in vitro fertilization: relevance to intracellular reactive oxygen species and glutathione contents.
Hashimoto S et al.
Molecular Reproduction and Development, 56, 520-520 (2000)
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Genome biology, 16, 66-66 (2015-04-19)
To understand the heterogeneous behaviors of individual cancer cells, it is essential to investigate gene expression levels as well as their divergence between different individual cells. Recent advances in next-generation sequencing-related technologies have enabled us to conduct a single-cell RNA-Seq
Influence of non-essential amino acids on apoptotic and necrotic death of mouse hybridoma cells in batch cultures
Fassnacht D
Biotechnology Letters, 19(1), 35-38 (1997)
Janis A Müller et al.
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Protocolos

This detailed procedure allows you to transduce Mouse Embryonic Fibroblasts (MEF) using MISSION ExpressMag Super Magnetic Kit.

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