E2758

β-Estradiol

BioReagent, powder, suitable for cell culture

• Sinônimo(s): 1,3,5-Estratriene-3,17β-diol, 17β-Estradiol, 3,17β-Dihydroxy-1,3,5(10)-estratriene, Dihydrofolliculin
• Fórmula empírica (Notação de Hill): C₁₈H₂₄O₂
• Número CAS: 50-28-2
• Peso molecular: 272.38
• Beilstein: 1914275
• Número CE: 200-023-8
• Número MDL: MFCD00003693
• Código UNSPSC: 12352209
• ID de substância PubChem: 24894483
• NACRES: NA.77

Propriedades

• fonte biológica: synthetic (organic)
• Nível de qualidade: 200
• linha de produto: BioReagent
• Ensaio: ≥98% (HPLC)
• forma: powder
• técnica(s): cell culture | mammalian: suitable; single cell analysis: suitable
• pf: 176-180 °C (lit.)
• solubilidade: ethanol: 50 mg/mL, clear, colorless
• Condições de expedição: ambient
• temperatura de armazenamento: room temp
• cadeia de caracteres SMILES: O[C@H]1CC[C@@]2([H])[C@]3([H])CCC4=CC(O)=CC=C4[C@@]3([H])CC[C@@]21C
• InChI: 1S/C18H24O2/c1-18-9-8-14-13-5-3-12(19)10-11(13)2-4-15(14)16(18)6-7-17(18)20/h3,5,10,14-17,19-20H,2,4,6-9H2,1H3/t14-,15-,16+,17+,18+/m1/s1
• chave InChI: VOXZDWNPVJITMN-ZBRFXRBCSA-N
• Informações sobre genes: human ... ESR1(2099) , ESR2(2100) , ESRRA(2101) , ESRRB(2103) , GPER(2852) , SERPINA6(866) ; mouse ... Esr1(13982) , Esr2(13983) , Esrra(26379) ; rat ... Afp(24177) , Ar(24208) , Esr1(24890) , Esr2(25149) , Shbg(24775)

Descrição

Descrição geral

β-Estradiol or estradiol-17 β (E2) is secreted majorly by the large preovulatory follicles in the ovary. In addition, estradiol can be synthesized de novo from cholesterol have androgens synthesized from cholesterol. The aromatization of androgen by enzyme aromatase (P450arom) results in the β-estradiol production.

Aplicação

β-Estradiol has been used:

• as a component of Dulbecco′s modified Eagle medium (DMEM) for culturing mammary tumor cells
• as a oestrogenic compound for testing neuroprotective effects
• as a component of medium199 for culturing oocytes

β-Estradiol is used to study cell differentiation and transformations (tumorigenicity).

Ações bioquímicas/fisiológicas

β-Estradiol (E2) is a major premenopausal hormone in women and is essential for spermiogenesis, sperm maturation and motility in men. Cultured bone marrow stem cells (BMSCs) have ability to synthesize E2 and could be exploited for treating estrogen deficiency. It mediates physiological functions through estrogen receptor (ER) α and ERβ.

The major estrogen secreted by the premenopausal ovary. Estrogens direct the development of the female phenotype in embryogenesis and during puberty by regulating gene transcription and, thus, protein synthesis. It also induces the production of gonadotropins which, in turn, induce ovulation. Exposure to estradiol increases breast cancer incidence and proliferation.

forma física

powder-RT; stock-frozen in working aliquots, avoid repeated freeze/thaw

Reconstituição

To prepare a 20 μg/ml stock solution, add 1 ml absolute ethanol to 1 mg β-estradiol; gently swirl to dissolve; add 49 ml sterile medium while mixing.

Informações de segurança

• Pictogramas: GHS08,GHS09
• Palavra indicadora: Danger
• Frases de perigo: H351,H360FD,H362,H410
• Declarações de precaução: P202 - P260 - P263 - P264 - P273 - P308 + P313
• Classificações de perigo: Aquatic Acute 1 - Aquatic Chronic 1 - Carc. 2 - Lact. - Repr. 1A
• Código de classe de armazenamento: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• Classe de risco de água (WGK): WGK 3
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable
• Equipamento de proteção individual: Eyeshields, Gloves, type P3 (EN 143) respirator cartridges