M3024

Meio mínimo essencial de Eagle

with Earle′s salts and non-essential amino acids, without L-glutamine, phenol red and sodium bicarbonate, Modified, powder, suitable for cell culture

• Sinônimo(s): MEM
• Código UNSPSC: 12352207
• NACRES: NA.75

Propriedades

• Nível de qualidade: 500
• forma: powder
• técnica(s): cell culture | mammalian: suitable
• componentes: Earle’s salts (5% CO₂): yes; L-glutamine: no; HEPES: no; NaHCO₃: no; sodium pyruvate: no; phenol red: no
• Condições de expedição: ambient
• temperatura de armazenamento: 2-8°C

Descrição

Descrição geral

Minimum Essential Medium Eagle contains 19 amino acids. The essential amino acids and the non-essential amino acids: L-ala, L-asn, L-asp, L-glu, L-gly, L-pro, and L-ser. This medium is preferred for the growth of adherent cells in the presence of reduced serum or as a base for the development of a serum-free medium. It is suitable for a wide variety of cells grown in monolayers.

Aplicação

Minimum Essential Medium Eagle has been used:

• to culture endometrial adenocarcinoma Ishikawa cells for bisphenol studies
• to culture MCF-7 cells for experimental treatments with hormone
• to maintain zebrafish pineal glands for action spectrum analysis

Características e benefícios

Contains high concentrations of amino acids so the medium resembles the protein composition of cultured mammalian cells

Quantidade

Formulated to contain 9.3 grams of powder per liter of medium.

Reconstituição

Supplement with 0.292 g/L L-glutamine, 2.2 g/L sodium bicarbonate.

Informações de segurança

• Código de classe de armazenamento: 13 - Non Combustible Solids
• Classe de risco de água (WGK): WGK 1
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable