D5030
Dulbecco′s Modified Eagle′s Medium
Without glucose, L-glutamine, phenol red, sodium pyruvate and sodium bicarbonate, powder, suitable for cell culture
Sinônimo(s):
DME, DMEM
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About This Item
Código UNSPSC:
12352207
NACRES:
NA.75
Produtos recomendados
Nível de qualidade
Formulário
powder
técnica(s)
cell culture | mammalian: suitable
componentes
phenol red: no
NaHCO3: no
L-glutamine: no
HEPES: no
glucose: no
sodium pyruvate: no
Condições de expedição
ambient
temperatura de armazenamento
2-8°C
Descrição geral
Dulbecco′s Modified Eagle′s Medium (DME) is a modification of Basal Medium Eagle (BME) that has been formulated with a 4-fold higher concentration of amino acids and vitamins. It includes additional supplementary components. The DME formula, first reported for culturing embryonic mouse cells, contained 1,000 mg/L of glucose. Modifying the medium with 4,500 mg/L glucose is optimal for culturing certain cell types.
The most basic formulation offered. This formulation is used by investigators who want to start with the essential components of DME, and have the flexibility to optimize the formula for their own application.
Aplicação
Dulbecco′s Modified Eagle′s Medium has been used:
- to culture the isolated flexor digitorum brevis (FDB) fibers to measure exogenous fatty acid (FA) utilization as part of oxygen consumption rate (OCR) measurements
- to culture the isolated tibialis anterior (TA) muscle fibers for lactate measurements
- to culture the human bone marrow mesenchymal stem cells for osteogenic differentiation to prepare a low-glucose medium to culture human hepatocellular carcinoma HepG2 cells
Quantidade
Formulated to contain 8.3 grams of powder per liter of medium.
Reconstituição
Supplement with 1.0 g/L glucose, 0.584 g/L L-glutamine, 3.7 g/L sodium bicarbonate.
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Palavra indicadora
Warning
Frases de perigo
Declarações de precaução
Classificações de perigo
Eye Irrit. 2
Código de classe de armazenamento
11 - Combustible Solids
Classe de risco de água (WGK)
WGK 3
Ponto de fulgor (°F)
Not applicable
Ponto de fulgor (°C)
Not applicable
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