D5546

DMEM - low glucose

With sodium bicarbonate and sodium pyruvate, without ʟ-glutamine, sterile liquid, suitable for cell culture

• Sinônimo(s): DME, DMEM
• Número MDL: MFCD00217342
• Código UNSPSC: 12352207
• NACRES: NA.75

Propriedades

• product name: Meio de Eagle modificado por Dulbecco - baixo teor de glicose, With 1000 mg/L glucose, and sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture
• esterilidade: sterile-filtered
• forma: liquid
• técnica(s): cell culture | mammalian: suitable
• Impurezas: endotoxin, tested
• componentes: NaHCO₃: yes; phenol red: yes; glucose: low; HEPES: no; sodium pyruvate: yes; L-glutamine: no
• Condições de expedição: ambient
• temperatura de armazenamento: 2-8°C

Descrição

Descrição geral

Dulbecco′s Modified Eagle′s Medium (DMEM) is a modification of Basal Medium Eagle (BME) that contains a higher concentration of amino acids, vitamins, and additional supplementary components. The original DMEM formula, first reported for culturing embryonic mouse cells, contained 1,000 mg/L of glucose. An alteration with 4,500 mg/L glucose is optimal in cultivating certain cell types. This modification has been shown to improve the stability of the medium. 

Aplicação

Dulbecco′s Modified Eagle′s Medium - low glucose has been used to culture:

• human osteosarcoma cells and HeLa cells
• primary human mesenchymal stromal cells (hBMSCs)
• human embryonic kidney cell lines

Reconstituição

Supplement with 0.584 g/L glutamine.

Informações de segurança

• Código de classe de armazenamento: 12 - Non Combustible Liquids
• Classe de risco de água (WGK): WGK 3
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable