M0268
Meio mínimo essencial de Eagle
With Earle′s salts and L-glutamine, without sodium bicarbonate, powder, suitable for cell culture
Sinônimo(s):
MEM
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About This Item
Código UNSPSC:
12352207
NACRES:
NA.75
Produtos recomendados
Nível de qualidade
forma
powder
técnica(s)
cell culture | mammalian: suitable
componentes
HEPES: no
phenol red: yes
Earle’s salts (5% CO2): yes
NaHCO3: no
L-glutamine: yes
sodium pyruvate: no
Condições de expedição
ambient
temperatura de armazenamento
2-8°C
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Descrição geral
Minimum Essential Medium Eagle (MEM) is one of the most widely used synthetic cell culture media. MEM has been used for culturing several cells grown in monolayers. It is commonly used to grow attached cell lines, such as fibroblasts, in the presence of fetal bovine sera (FBS), calf, or horse sera.
Aplicação
Minimum Essential Medium Eagle has been used:
- in the isolation and culture of primary hepatocytes
- to culture the human endometrial carcinoma cells Ishikawa (ATTC) and L-Wnt-3a cells (ATCC) that secrete Wnt family member 3a (Wnt-3a) ligand
- to culture the AN3CA (endometrial cancer cell line) cells, and HEC1-B human (endometrial adenocarcinoma cell line) cells
Características e benefícios
- Minimum Essential Medium Eagle (MEM) contains higher concentrations of amino acids to mimic the protein composition of cultured mammalian cells.
- The formulation has been altered by optional elimination of calcium to enable the growth of cells in suspension culture.
Quantidade
Formulated to contain 9.5 grams of powder per liter of medium.
Reconstituição
Supplement with 2.2 g/L sodium bicarbonate.
Outras notas
Essentially the same as the original formula, except it is provided as a powder.
geralmente comprado junto com este produto
Nº do produto
Descrição
Preços
Código de classe de armazenamento
13 - Non Combustible Solids
Classe de risco de água (WGK)
WGK 2
Ponto de fulgor (°F)
Not applicable
Ponto de fulgor (°C)
Not applicable
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