M0275

Meio mínimo essencial de Eagle

With Earle′s salts, without ʟ-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture, 10x

• Sinônimo(s): MEM
• Código UNSPSC: 12352207
• NACRES: NA.71

Propriedades

• product name: Meio mínimo essencial de Eagle, 10 ×, With Earle′s salts, without L-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture
• esterilidade: sterile-filtered
• forma: liquid
• concentração: 10 ×
• técnica(s): cell culture | mammalian: suitable
• Impurezas: endotoxin, tested
• componentes: phenol red: yes; HEPES: no; Earle’s salts (5% CO₂): yes; L-glutamine: no; sodium pyruvate: no; NaHCO₃: no
• Condições de expedição: ambient
• temperatura de armazenamento: 2-8°C

Descrição

Aplicação

Generally used to grow attached cell lines, such as fibroblasts, in the presence of FBS, calf or horse sera. The L-glutamine and sodium bicarbonate are added to the medium after dilution to 1x and pH adjustment.

Minimum Essential Medium Eagle has been used for the formulation of collagen methacrylamide (hydrogel). It has also been used to maintain articular cartilage of bovine knee joints, which was later used for MRI (magnetic resonance imaging) study and histological analysis.

Reconstituição

Supplement with 0.292 g/L L-glutamine, 2.2 g/L sodium bicarbonate at 1×.

Informações de segurança

• Código de classe de armazenamento: 12 - Non Combustible Liquids
• Classe de risco de água (WGK): WGK 3
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable