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Key Documents

S8761

Sigma-Aldrich

Sodium Bicarbonate

Solution (7.5%), sterile-filtered, suitable for cell culture

Sinônimo(s):

aqueous sodium bicarbonate

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About This Item

Número CAS:
Número MDL:
Código UNSPSC:
12161702
ID de substância PubChem:
NACRES:
NA.75

product name

Sodium bicarbonate solution, solution (7.5%), sterile-filtered, BioReagent, suitable for cell culture

esterilidade

sterile-filtered

Nível de qualidade

linha de produto

BioReagent

forma

solution (7.5%)

técnica(s)

cell culture | mammalian: suitable

Impurezas

endotoxin, tested

pH

7.8-8.2

temperatura de armazenamento

2-8°C

cadeia de caracteres SMILES

[Na+].OC([O-])=O

InChI

1S/CH2O3.Na/c2-1(3)4;/h(H2,2,3,4);/q;+1/p-1

chave InChI

UIIMBOGNXHQVGW-UHFFFAOYSA-M

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Descrição geral

Sodium bicarbonate (NaHCo3) is a commonly used alkaline buffering agent(5) in laboratories. The addition of this buffer to the cell culture medium aids in maintaining the appropriate pH by using the carbon dioxide present in the incubator. In addition to its buffering properties, Sodium bicarbonate also acts as a source of sodium and aids in sodium gluconate synthesis during the fermentation process.

Aplicação

Sodium bicarbonate solution has been used:
  • as a component of Hanks′ buffered salt solution (HBSS) and recording medium for culturing of tissue explants from rats or mice
  • as a component of Dulbecco′s modified Eagle′s medium (DMEM) to culture mice tissue explants
  • as a component of Minimum Essential Media (MEM) to study the cytotoxicity against human colorectal adenocarcinoma cells and mammary gland adenocarcinoma
  • to prepare collagen mix droplets for 3D collagen sprouting assay
  • to prepare collagen matrix for rat epidermal keratinocyte (REK) organotypic culture

Nota de preparo

Prepared in cell culture grade water.

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Código de classe de armazenamento

12 - Non Combustible Liquids

Classe de risco de água (WGK)

WGK 1

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable


Certificados de análise (COA)

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Protocolos

3-D Culture Matrix™ Collagen I may be used as a gel on which to grow cells or a media additive alone or in concert with other basement membrane components to study cellular growth and differentiation in three dimensions in vitro.

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