M4642
Meio mínimo essencial de Eagle
With Hanks′ salts and ʟ-glutamine, without sodium bicarbonate, powder, suitable for cell culture
Sinônimo(s):
MEM
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About This Item
Código UNSPSC:
12352207
NACRES:
NA.75
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Nome do produto
Meio mínimo essencial de Eagle, With Hanks′ salts and L-glutamine, without sodium bicarbonate, powder, suitable for cell culture
Formulário
powder
técnica(s)
cell culture | mammalian: suitable
componentes
glucose: yes
Hanks’ salts (2% CO2): yes
L-glutamine: yes
Earle’s salts (5% CO2): no
NaHCO3: no
phenol red: yes
sodium pyruvate: no
stable glutamine: no
HEPES: no
Condições de expedição
ambient
temperatura de armazenamento
2-8°C
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Descrição geral
Minimum Essential Medium Eagle (MEM) is a synthetic cell culture media, developed by Harry Eagle. It has high concentrations of amino acids, so the medium closely approximates the protein concentration of cultured mammalian cells. Optional supplementation of non-essential amino acids to the formulations that incorporate either Hanks′ or Earle′s salts has increased the applicability of this medium. This medium lacks calcium that permits the growth of cells in suspension. Modification of the original MEM formulation. Earle′s salts have been replaced with Hanks′ salts. Since the bicarbonate concentration is substantially lower, this medium has a substantially reduced buffering capacity.
Aplicação
Minimum Essential Medium Eagle (MEM) has been used:
- as a component of the Hank′s MEM (HMEM)-Pipes buffer to induce anaphylatoxin induced-ear swelling in mice
- as a component of the slice culture medium (SCM) for the incubation of mouse hippocampal slice cultures
- to culture Müller cells from guinea pigs
Quantidade
Formulated to contain 10.6 grams of powder per liter of medium.
Reconstituição
Supplement with 0.35 g/L sodium bicarbonate.
geralmente comprado junto com este produto
Nº do produto
Descrição
Preços
Código de classe de armazenamento
11 - Combustible Solids
Classe de risco de água (WGK)
WGK 1
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