M5650
Meio mínimo essencial de Eagle
With non-essential amino acids, Earle′s salts and sodium bicarbonate, without ʟ-glutamine, liquid, sterile-filtered, suitable for cell culture
Sinônimo(s):
MEM
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About This Item
Código UNSPSC:
12352207
NACRES:
NA.75
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Nome do produto
Meio mínimo essencial de Eagle, With Earle′s salts, non-essential amino acids and sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture
Nível de qualidade
esterilidade
sterile-filtered
Formulário
liquid
técnica(s)
cell culture | mammalian: suitable
Impurezas
endotoxin, tested
componentes
phenol red: yes
NaHCO3: yes
L-glutamine: no
sodium pyruvate: no
HEPES: no
Earle’s salts (5% CO2): yes
Condições de expedição
ambient
temperatura de armazenamento
2-8°C
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Descrição geral
Minimum Essential Medium Eagle (MEM) is a synthetic cell culture media, developed by Harry Eagle. It has higher concentrations of amino acids so the medium more closely approximates the protein composition of cultured mammalian cells. Optional supplementation of non-essential amino acids to the formulations that incorporate either Hanks′ or Earle′s salts has enhanced the uses of this medium. This formulation has been further modified by the optional elimination of calcium to permit the growth of cells in suspension culture. MEM can be used for the cultivation of a wide variety of cells grown in monolayers.
Aplicação
Minimum Essential Medium Eagle has been used to culture:
This medium may be preferred for the growth of adherent cells in the presence of reduced serum or as a base for the development of a serum-free medium.
- HeLa cells
- Madin-Darby canine kidney (MDCK) cells
- chondrosarcoma cell line
This medium may be preferred for the growth of adherent cells in the presence of reduced serum or as a base for the development of a serum-free medium.
Reconstituição
Supplement with 0.292 g/L L-glutamine.
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Código de classe de armazenamento
11 - Combustible Solids
Classe de risco de água (WGK)
WGK 1
Ponto de fulgor (°F)
Not applicable
Ponto de fulgor (°C)
Not applicable
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