CHY5S

α-キモトリプシン ウシ膵臓由来

≥40 units/mg protein, vial of 5 mg

• 別名: α-chymotrypsin A and B
• CAS番号: 9004-07-3
• Enzyme Commission number: 3.4.21.1 (BRENDA, IUBMB)
• EC Number: 232-671-2
• MDL番号: MFCD00130481
• UNSPSCコード: 12352204
• NACRES: NA.54

特性

• 由来生物: bovine pancreas
• 品質水準: 200
• 詳細: aseptically filled
• タイプ: Type IV-S
• フォーム: solid
• 比活性: ≥40 units/mg protein
• 分子量: 25 kDa
• 組成: protein, ≥85% UV
• 包装: vial of 5 mg
• UniProtアクセッション番号: P00767
• 保管温度: −20°C
• 遺伝子情報: cow ... CTRB1(618826)

詳細

アプリケーション

α-Chymotrypsin from bovine has been used in a study to inform proteasome inhibition in order to advance anticancer research. α-Chymotrypsin from bovine has also been used in a study that functionalized surface anchored poly(methylhydrosiloxane) thin films on oxidized silicon wafers.

α-Chymotrypsin from bovine pancreas has been used:

• as a supplement for the collection of semen into Tris diluent
• as one of the proteases in the analysis of major histocompatibility complex (MHC) class II protease sensitivity assay
• as a component in YEPD broth for biofilm dispersion assay
• in the preparation of chitinase–chymotrypsin–DMSO buffer (CCD buffer) for enzymatic digestion of larvae

The enzyme from Sigma has been used to assess the effect of limited proteolysis with α-chymotrypsin on the sperm penetration.

生物化学的／生理学的作用

結合のカルボキシル基側にに芳香族側鎖または大きな疎水性側鎖(チロシン, トリプトファン, フェニルアラニン, メチオニン)を持つペプチド結合を加水分解するセリンプロテア-ゼです。

α-Chymotrypsin is a serine peptidase and has 241 amino acid residues contained in three polypeptide chains (A chain-13 residues, B chain-131 residues, and C chain-97 residues) linked by disulfide bridges. Molecular weight of this enzyme is found to be 25 kDa. Its pI is 8.75. It selectively hydrolyzes peptide bonds on the C-terminal side of tyrosine, phenylalanine, tryptophan, and leucine. Ca²⁺ activates and stabilizes the enzyme. The enzyme is inhibited by diisopropyl fluorophosphate (DFP), phenylmethanesulfonyl fluoride (PMSF), N-p-tosyl-L-phenylalanine chloromethyl ketone (TPCK), chymostatin, aprotinin, α1-antitrypsin, and α2-macroglobulin, 10 mM Cu²⁺ and Hg^2+.

単位の定義

1ユニットは、pH 7.8、25°Cで1分間あたり1.0 μmolのBTEEを加水分解します。

アナリシスノート

タンパク質はA1%/280で測定。

安全性情報

• ピクトグラム: GHS08,GHS07,GHS09
• シグナルワード: Danger
• 危険有害性情報: H302,H315,H319,H334,H335,H400
• 注意書き: P261 - P264 - P273 - P301 + P312 - P302 + P352 - P305 + P351 + P338
• 危険有害性の分類: Acute Tox. 4 Oral - Aquatic Acute 1 - Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
• ターゲットの組織: Respiratory system
• 保管分類コード: 11 - Combustible Solids
• WGK: WGK 1
• 個人用保護具 (PPE): dust mask type N95 (US), Eyeshields, Faceshields, Gloves