P8250
Peroxidase
Type II, essentially salt-free, lyophilized powder, 150-250 units/mg solid (using pyrogallol)
Sinônimo(s):
Doador:peróxido de hidrogênio oxidorredutase, Peroxidase de raiz forte
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About This Item
Produtos recomendados
fonte biológica
horseradish
Nível de qualidade
tipo
Type II
Formulário
essentially salt-free, lyophilized powder
atividade específica
150-250 units/mg solid (using pyrogallol)
peso molecular
~44 kDa
solubilidade
0.1 M phosphate buffer: soluble (pH 6.0)
H2O: soluble
razão de absorbância
RZ ≥1.8
aplicação(ões)
diagnostic assay manufacturing
temperatura de armazenamento
2-8°C
cadeia de caracteres SMILES
[O+H2]O[O-]
InChI
1S/H2O3/c1-3-2/h1-2H
chave InChI
JSPLKZUTYZBBKA-UHFFFAOYSA-N
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Descrição geral
A peroxidase de raiz forte é isolada da raiz de Amoracia rusticana e pertence ao grupo ferroprotoporfirínico de peroxidases. A HRP é um polipeptídeo de cadeia simples com quatro pontes dissulfeto. É uma glicoproteína que contém 18% de carboidratos. A composição de carboidratos consiste em galactose, arabinose, xilose, fucose, manose, manosamina e galactosamina, dependendo da isoenzima específica. O peso molecular (~44 kDa) inclui a cadeia polipeptídica (33.890 Daltons), hemina mais Ca2+ (~700 Daltons) e carboidratos (~9.400 Daltons). Existem no mínimo sete isoenzimas de HRP. O ponto isoelétrico das isoenzimas de peroxidase de raiz forte varia entre 3,0 e 9,0.
Aplicação
Horseradish peroxidase (HRP) is isolated from horseradish roots (Amoracia rusticana). It is used in biochemistry applications such as western blots, ELISA and Immunohistochemistry. Horseradish peroxidase is used to amplify a weak signal and increase detectability of a target molecule, such as a protein. Horseradish peroxidase, product P8250, has been used to study nonoral antigens in inflamed gingiva and Ebola virus glycoprotein toxicity.
The enzyme has been used as a comparison during the peroxidase assay of extract from mature tall fescue leaf blades. It has also been used to measure H2O2 production.
Ações bioquímicas/fisiológicas
HRP readily combines with hydrogen peroxide (H2O2) and the resultant [HRP-H2O2] complex can oxidize a wide variety of hydrogen donors. The optimal pH is 6.0-6.5 and the enzyme is most stable in the pH range of 5.0-9.0. HRP can be conjugated to antibodies by several different methods including glutaraldehyde, periodate oxidation, through disulfide bonds, and also via amino and thiol directed cross-linkers. It is smaller and more stable than the enzyme labels β-galactosidase and alkaline phosphatase. Hence, it is the most desired label. Also, its glycosylation leads to lower non-specific binding. It is also used for the determination of glucose and peroxides in solution. Sodium azide, cyanide, L-cystine, dichromate, ethylenethiourea, hydroxylamine, sulfide, vanadate, p-aminobenzoic acid, and Cd2+, Co2+, Cu2+, Fe3+, Mn2+, Ni2+, Pb2+ ions are found to inhibit the enzyme activity.
When incubated with a substrate, horseradish peroxidase produces a coloured, fluorimetric, or luminescent derivative of the labeled molecule, allowing quantification. Horseradish peroxidase has been shown to slightly reduce the level of inhibition in a cydAB mutant.
Qualidade
Preliminary work shows it to contain at least five isoenzymes.
Definição da unidade
Uma unidade de pirogalol forma 1,0 mg de purpurogalina a partir do pirogalol em 20 segundos em pH 6,0 a 20 °C.
Nota de análise
RZ (Reinheitszahl) é a taxa de absorbância A403/A275 determinada a 0,5-1,0 mg/ml em água deionizada. É uma medida do teor de hemina, e não da atividade enzimática. Mesmo preparações com RZ alto podem ter atividade enzimática baixa.
Outras notas
Veja mais informações sobre peroxidase em www.sigma-aldrich.com/enzymeexplorer.
Inibidor
Nº do produto
Descrição
Preços
substrato
Nº do produto
Descrição
Preços
Palavra indicadora
Danger
Frases de perigo
Declarações de precaução
Classificações de perigo
Resp. Sens. 1
Código de classe de armazenamento
13 - Non Combustible Solids
Classe de risco de água (WGK)
WGK 1
Equipamento de proteção individual
Eyeshields, Gloves, type N95 (US)
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J W Macadam et al.
Plant physiology, 99(3), 872-878 (1992-07-01)
Cessation of cell expansion has been associated with cell wall cross-linking reactions catalyzed by peroxidase. This study utilized two genotypes of tall fescue (Festuca arundinacea Schreb.) that differ in length of the leaf elongation zone to investigate the relationship between
Chuan Chiang-Ni et al.
Journal of microbiology, immunology, and infection = Wei mian yu gan ran za zhi, 49(6), 837-842 (2015-02-05)
The phenotypic heterogeneity of the human pathogen Streptococcus pyogenes [group A streptococcus (GAS)] is associated with bacterial virulence variation. During invasive GAS infection, mutations in the two-component regulatory system covR/covS leads to increases in hyaluronic acid capsule production, virulence genes
S M Mallison et al.
Infection and immunity, 56(4), 823-830 (1988-04-01)
In vitro experimentation indicates that periodontitis-associated bacteria contain potent polyclonal B-cell activators (PBA). We reasoned that if PBA were operative in vivo, plasma cells specific for nonoral antigens should be present in the inflamed gingival tissues, which are characterized by
W E Bullock et al.
The Journal of experimental medicine, 165(1), 195-210 (1987-01-01)
The principal host cell of H. capsulatum (Hc) is the M phi within which the pathogenic yeast phase of the fungus multiplies during active disease. The initial interaction between Hc yeasts and M phi therefore is a crucial step in
Nancy J Sullivan et al.
Journal of virology, 79(1), 547-553 (2004-12-15)
Ebola virus infection causes a highly lethal hemorrhagic fever syndrome associated with profound immunosuppression through its ability to induce widespread inflammation and cellular damage. Though GP, the viral envelope glycoprotein, mediates many of these effects, the molecular events that underlie
Protocolos
Enzymatic Assay of Diamine Oxidase (E.C. No. 1.4.3.22)
This procedure may be used for all Alcohol Oxidase products.
Enzymatic Assay of Cholesterol Oxidase
Reinheitszahl (RZ) is the ratio of absorbance due to hemin (A403, Soret region) to absorbance due to protein (A275).
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