10814407001

Peroxidase (POD)

EIA grade, from horseradish

• Sinônimo(s): POD
• Número da licença da enzima: 1.11.1.7 (BRENDA, IUBMB)
• Código UNSPSC: 12352204

Propriedades

• fonte biológica: horseradish
• Nível de qualidade: 100
• Ensaio: 90% (HPLC)
• forma: lyophilized (salt-free)
• atividade específica: ~225 U/mg lyophilizate, 25 °C (Guaiacol, H2O2)
• embalagem: pkg of 100 mg
• fabricante/nome comercial: Roche
• concentração: 10 g/L
• técnica(s): activity assay: suitable
• cor: red to brown
• pH ideal: 6.0-6.5
• solubilidade: water: soluble at 68 °F
• adequação: suitable for molecular biology
• nº de adesão UniProt: P00433
• aplicação(ões): detection; life science and biopharma
• atividade externa: ATPase <0.00100%, none detected; AcP <0.00100%; Catalase <0.700%
• Condições de expedição: dry ice
• temperatura de armazenamento: −20°C (−15°C to −25°C)

Descrição

Descrição geral

Donor: hydrogen-peroxide oxidoreductase
Peroxidase can be coupled to other proteins via its amino groups, as well as its carbohydrate moiety. The reconstituted solution can be used directly for conjugation without prior dialysis.

Aplicação

Peroxidase (POD) has been used in the spermine oxidase enzyme activity assay.

Sequência

Blocking substances
Preparations does not contain substances that block the amino groups, but in contrast to other enzymes POD has only 2 to 3 free amino groups which are not accessible; even by extended dialysis of POD, the number of NH₂-groups/mol does not change.

POD
POD contains 1 molecule of protoheme IX as prosthetic group and 2 molecules of Ca²⁺.
POD exists in > 10 isoenzymic forms (some sources estimate approx. 40 isoenzymes).

Carbohydrate Content
12 to 14.5 mg/100 mg Iyophilizate

Free Amino Groups
Free amino groups: 2 to 3 mol/mol enzyme

Nota de preparo

Activator: Ammonia, pyridine, imidazole, all at pH >7; detergents
Working concentration: A suggested working ratio, for immediate use in coupling procedures, is 2 mg POD to 4 mg lgG (molar ratio = 2:1, POD:IgG).
Specific activity: approximately 225U/mg lyophilizate (+25°C, guaiacol, H₂O₂) approximately 1,000 U/mg lyophilizate (+25°C, ABTS, H₂O₂, pH 5.0) approximately 3,500 U/mg lyophilizate (+25°C, tetramethylbenzidine, H₂O₂)
Amino groups: 2 - 3moles/mole enzyme

Carbohydrates: 12.0 - 14.5% (w/w)
Isoenzymes: ≥90% isoenzyme C (HPLC)
Purity: single peak (HPLC)
Purity number: 3.0 - 3.5 (A₄₀₃/A₂₇₅)

Armazenamento e estabilidade

Keep container tightly closed in a dry and well-ventilated place.

Outras notas

For life science research only. Not for use in diagnostic procedures.

Informações de segurança

• Pictogramas: GHS08
• Palavra indicadora: Danger
• Frases de perigo: H317,H334
• Declarações de precaução: P261 - P280 - P284 - P304 + P340 - P333 + P313 - P342 + P311
• Classificações de perigo: Resp. Sens. 1 - Skin Sens. 1
• Código de classe de armazenamento: 11 - Combustible Solids
• Classe de risco de água (WGK): WGK 1
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable