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Merck
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Documentos Principais

11089153001

Roche

Streptavidin-POD conjugate

liquid, pkg of 1 mL

Sinônimo(s):

streptavidin

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About This Item

Código UNSPSC:
12352200

Formulário

liquid

Nível de qualidade

embalagem

pkg of 1 mL

fabricante/nome comercial

Roche

temperatura de armazenamento

-10 to -25°C

Descrição geral

Streptavidin is covalently coupled to horseradish peroxidase. The conjugate is used for detection of biotin-labelled substances.

Aplicação

Streptavidin-POD has been used for:
  • ELISA
  • In situ hybridization
  • Western blot
It is also used for immunohistochemistry.

Definição da unidade

One unit Streptavidin-POD, corresponding to one unit peroxidase, will oxidize 1 μmol of guaiacol tetramer in one minute at +25 °C and pH 7.0.

Unit Conversion: 1 U (+25 °C; guaiacol as substrate; pH7.0) corresponds to 1.24 U (+25 °C; 1.7 mM ABTS as substrate; pH= 6.0) or to 4.0 U (+25 °C; 8.7 mM ABTS as substrate; pH = 5.0).

forma física

Liquid, stabilized

Nota de preparo

Working concentration: 5 - 500 mU/ml (i.e., stock solution can be diluted).Working concentration of conjugate depends on application and substrate. The following concentrations should be taken as a guideline:
  • ELISA: 50 to 200 mU/ml
  • Immunohistocytochemistry: 250 to 1000 mU/ml
  • Western blot: 200 to 500 mU/ml

Outras notas

For life science research only. Not for use in diagnostic procedures.

Pictogramas

Exclamation mark

Palavra indicadora

Warning

Frases de perigo

Classificações de perigo

Skin Sens. 1

Código de classe de armazenamento

11 - Combustible Solids

Classe de risco de água (WGK)

WGK 1

Ponto de fulgor (°F)

does not flash

Ponto de fulgor (°C)

does not flash


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Effective vaccination against tumour-associated antigens (TAA) such as the 5T4 oncofoetal glycoprotein may be limited by the nature of the T cell repertoire and the influence of immunomodulatory factors in particular T regulatory cells (Treg). Here, we identified mouse 5T4-specific
Angelika Hammerer-Lercher et al.
Clinical chemistry, 54(5), 858-865 (2008-03-15)
The specific forms of pro-B-type natriuretic peptide (proBNP) that occur in human blood are not yet clear. We demonstrated the presence of several proBNP forms in human plasma with a new affinity chromatography method that can be used in combination
Triple-Labeling Whole-Mount In Situ Hybridization Method for Analysis of Overlapping Gene Expression in Brain Tissue with High Level of Autofluorescence.
Chen J
Journal of Cytology & Histology, http://dx-http://dx (2015)
Benjamin Meyer et al.
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To comprehensively evaluate SARS-CoV-2 specific B-cell and antibody responses up to one year after mild COVID-19. In 31 mildly symptomatic COVID-19 participants SARS-CoV-2-specific plasmablasts and antigen-specific memory B cells were measured by ELISpot. Binding antibodies directed against the proteins spike
Husheem Michael et al.
Frontiers in immunology, 11, 196-196 (2020-03-03)
Human rotavirus (HRV) is a leading cause of morbidity and mortality in children, especially in developing countries. Malnutrition is prevalent in these countries, which may contribute to the decreased oral vaccine efficacy, posing a concern for global health. Neonatal gnotobiotic

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