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F5803

Sigma-Aldrich

o-Dianisidine dihydrochloride

Suitable for use in glucose determination

Sinônimo(s):

3,3′-Dimethoxybenzidine dihydrochloride, Fast Blue B

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About This Item

Fórmula empírica (Notação de Hill):
C14H16N2O2 · 2HCl
Número CAS:
20325-40-0
Peso molecular:
317.21
Beilstein:
3917996
Número CE:
243-737-5
Número MDL:
MFCD00012488
Código UNSPSC:
12171500
ID de substância PubChem:
24894894
NACRES:
NA.47

forma

crystalline

faixa de pH

6.95-7.05

pf

268 °C

solubilidade

water: 2.5 mg/mL, colorless

aplicação(ões)

diagnostic assay manufacturing
hematology
histology

temperatura de armazenamento

2-8°C

cadeia de caracteres SMILES

Cl.Cl.COc1cc(ccc1N)-c2ccc(N)c(OC)c2

InChI

1S/C14H16N2O2.2ClH/c1-17-13-7-9(3-5-11(13)15)10-4-6-12(16)14(8-10)18-2;;/h3-8H,15-16H2,1-2H3;2*1H

chave InChI

UXTIAFYTYOEQHV-UHFFFAOYSA-N

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Descrição geral

A preweighed vial containing 50 mg o-Dianisidine.

Especificidade

o-Dianisidine (3,3′-dimethoxybenzidine) is a peroxidase substrate suitable for use in ELISA procedures. This substrate produces a soluble end product that is yellow-orange in color and can be read spectrophotometrically at 405 nm. The reaction may be stopped with 5 M HCl.

Aplicação

o-Dianisidine dihydrochloride is suitable for use in the glucose determination. It is suitable reagent used in the following studies:
  • As substrate for the determination of serum ceruloplasmin from its oxidase activity.
  • Determination of total antioxidant response (TAR) against potent free radical reactions by a novel, colorimetric and fully automated method.
  • As colorimetric indicator for the simultaneous semi-quantitative estimation of glucose, lactate and uric acid on paper-based microfluidic devices (microPAD).
  • Myeloperoxidase activity assay.

Advertência

Probably carcinogenic.

enzima

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Descrição
Preços

padrão

Nº do produto
Descrição
Preços

Palavra indicadora

Danger

Frases de perigo

H302,H314,H350

Classificações de perigo

Acute Tox. 4 Oral - Carc. 1B - Eye Dam. 1 - Skin Corr. 1

Código de classe de armazenamento

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

Classe de risco de água (WGK)

WGK 3

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

Equipamento de proteção individual

Eyeshields, Gloves, type P3 (EN 143) respirator cartridges

Certificados de análise (COA)

Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.

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Wijitar Dungchai et al.
Analytica chimica acta, 674(2), 227-233 (2010-08-04)
We report here the use of multiple indicators for a single analyte for paper-based microfluidic devices (microPAD) in an effort to improve the ability to visually discriminate between analyte concentrations. In existing microPADs, a single dye system is used for
Measurement of ceruloplasmin from its oxidase activity in serum by use of o-dianisidine dihydrochloride.
K H Schosinsky et al.
Clinical chemistry, 20(12), 1556-1563 (1974-12-01)
W Shi et al.
Journal of dairy science, 102(4), 3082-3096 (2019-02-11)
The objective of this study was to evaluate the effects of supplementing a Saccharomyces cerevisiae fermentation product (SCFP; NutriTek, Diamond V, Cedar Rapids, IA) during the periparturient period (d -28 ± 3 to 44 ± 3 relative to calving) on
Risheng Ye et al.
The Journal of clinical investigation, 128(3), 1178-1189 (2018-02-20)
The compensatory proliferation of insulin-producing β cells is critical to maintaining glucose homeostasis at the early stage of type 2 diabetes. Failure of β cells to proliferate results in hyperglycemia and insulin dependence in patients. To understand the effect of
L O Chow et al.
Journal of dairy science, 91(4), 1534-1543 (2008-03-20)
Objectives of the study were to evaluate the effect of planting date on in vitro neutral detergent fiber digestibility (IVFD) of whole-crop barley (Hordeum vulgare) and its effects on productivity of lactating dairy cows. Two cultivars of barley were planted
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Protocolos

Enzymatic Assay of Diamine Oxidase (E.C. No. 1.4.3.22)

Enzymatic Assay of Diamine Oxidase (E.C. No. 1.4.3.22)

Enzymatic Assay of Glucose Oxidase

Enzymatic Assay of Glucose Oxidase

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