P2645
Protein Kinase A Catalytic Subunit from bovine heart
≥9 units/μg protein (cyclic-AMP is not required for this activity), lyophilized (white powder to sticky mass to hard pellet)
Sinônimo(s):
PKA
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About This Item
Número MDL:
Código UNSPSC:
12352204
eCl@ss:
32160410
NACRES:
NA.32
Produtos recomendados
forma
lyophilized (white powder to sticky mass to hard pellet)
Nível de qualidade
atividade específica
≥9 units/μg protein (cyclic-AMP is not required for this activity)
peso molecular
40,862 Da
temperatura de armazenamento
−20°C
Descrição geral
Protein Kinase A enzyme is composed of two subunits- catalytic and regulatory. The catalytic subunit exists as a monomer in the presence of cAMP and has a molecular weight of 40,862 Da.
Aplicação
Protein Kinase A (PKA) Catalytic Subunit from bovine heart has been used-
- to study PKA-mediated inhibition of IRK1 (inwardly rectifying K+) channels
- in in Vitro PKA pPhosphorylation assay
- in Vitro affinity binding assays
- to study effects of PKA on inspiratory drive currents in functionally active motorneurons
Ações bioquímicas/fisiológicas
Protein Kinase A (PKA) controls the transduction of Hedgehog signaling and participates in proliferation and fate specification. It phosphorylates several neurotransmitter receptors, transcription factors and constituents of various intracellular signaling pathways.
Protein Kinase A catalyzes the transfer of terminal phosphate from ATP to threonine or serine residues present on various proteins. This protein is inactive in the absence of cAMP, where the catalytic and regulatory subunits are bound together. The regulatory subunit, in the presence of cAMP, binds to cAMP and releases the catalytic subunit.
Embalagem
Package size based on phosphorylating units
Definição da unidade
Phosphorylating Activity: One unit will transfer 1.0 picomole phosphate from ATP to hydrolyzed and partially dephosphorylated casein per minute at pH 6.5 at 30°C, determined by measuring the production of ADP.
forma física
Lyophilized powder with sucrose and phosphate buffer salts as stabilizer.
Nota de preparo
Prepared from protein kinase A (P 5511)
Exoneração de responsabilidade
Please note that the pack size has been changed to align with the unit definition, while the number of phosphorylating units remain the same as before.
inibidor
Nº do produto
Descrição
Preços
Código de classe de armazenamento
11 - Combustible Solids
Classe de risco de água (WGK)
WGK 3
Ponto de fulgor (°F)
Not applicable
Ponto de fulgor (°C)
Not applicable
Equipamento de proteção individual
Eyeshields, Gloves, type N95 (US)
Certificados de análise (COA)
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