C8482
Protein Kinase A catalytic subunit human
>90% (SDS-PAGE), recombinant, expressed in E. coli, buffered aqueous glycerol solution
Sinônimo(s):
Cyclic AMP-dependent protein kinase catalytic subunit, histidine-tagged, recombinant human
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About This Item
Produtos recomendados
recombinante
expressed in E. coli
Nível de qualidade
Ensaio
>90% (SDS-PAGE)
Formulário
buffered aqueous glycerol solution
atividade específica
>1000 units/mg protein
peso molecular
43.5 kDa
nº de adesão UniProt
Condições de expedição
dry ice
temperatura de armazenamento
−70°C
Informações sobre genes
human ... PRKACA(5566)
Ações bioquímicas/fisiológicas
Phosphorylates a spectrum of substrates, and regulates many cellular processes.
Ubiquitous serine-threonine kinase that phosphorylates a broad spectrum of substrates, and regulates many cellular processes. The catalytic subunit is released following binding of cyclic AMP to the regulatory subunits of the PKA holoenzyme. The free catalytic subunit has intrinsic activity and does not require added cyclic AMP.
forma física
Solution in 30 mM potassium phosphate buffer, pH 7.4, containing 50% glycerol, 150 mM KCl, 1 mM EDTA, and 1 mM DTT.
Nota de análise
One unit will transfer 1 nanomole of phosphate from 32P-ATP to kemptide substrate per minute at pH 7.4 at 30 °C (measured by a radioactive filter-binding assay).
Km (ATP) = 25 μM at 30 °C, pH 7.4. Km (kemptide) = 42 μM (33 μg/ml) at 30 °C, pH 7.4.
Km (ATP) = 25 μM at 30 °C, pH 7.4. Km (kemptide) = 42 μM (33 μg/ml) at 30 °C, pH 7.4.
Código de classe de armazenamento
10 - Combustible liquids
Classe de risco de água (WGK)
WGK 1
Ponto de fulgor (°F)
Not applicable
Ponto de fulgor (°C)
Not applicable
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