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Documentos Principais

MS0100

Supelco

ProteoMass Guanidination Kit

For improving MALDI-MS sensitivity and peptide sequence coverage

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About This Item

Código UNSPSC:
12352200
NACRES:
NA.24

Nível de qualidade

linha de produto

ProteoMass

aplicação(ões)

cleaning products
cosmetics
flavors and fragrances
food and beverages
personal care

compatibilidade

for use with (Complex cell extracts, pure protein solution, 1D and 2D PAGE gels)

Aplicação

The ProteoMass Guanidination Kit allows you to enhance MALDI-MS sensitivity, increase sequence coverage, and identify peptides with greater confidence. Following proteolytic digestion, peptides with C-terminal Arginine residues are ionized preferentially over peptides with C-terminal Lysine residues, leading to compromised sequence coverage and limited confidence during peptide mass fingerprint analysis. The ProteoMass Guanidination Kit efficiently and conveniently converts C-terminal Lysine residues to homoarginine, increasing MALDI signal strength and producing enhanced sequence coverage.

Características e benefícios

  • Identify more samples with greater accuracy and confidence
  • Increase throughput and save time - only 35 minutes to use the kit vs. 2 hours using traditional methods
  • Compatibility - compatible with 1D or 2D PAGE gel bands or spots, as well as complex cell extracts

Outras notas

One MS0100 kit is sufficient for 96 reactions.

Informações legais

ProteoMass is a trademark of Sigma-Aldrich Co. LLC

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Palavra indicadora

Danger

Frases de perigo

Classificações de perigo

Aquatic Acute 1 - Aquatic Chronic 3 - Eye Dam. 1 - Skin Corr. 1A - STOT SE 3

Órgãos-alvo

Respiratory system

Código de classe de armazenamento

8A - Combustible corrosive hazardous materials

Classe de risco de água (WGK)

WGK 3

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable


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Yoshimi Kanie et al.
PloS one, 4(5), e5434-e5434 (2009-05-06)
A variety of N-glycans attached to protein are known to involve in many important biological functions. Endoplasmic reticulum (ER) and Golgi localized enzymes are responsible to this template-independent glycan synthesis resulting glycoforms at each asparagine residues. The regulation mechanism such
Fumihiro Oshita et al.
Oncology reports, 24(3), 637-645 (2010-07-29)
To investigate the early changes in protein function that induce micro-metastasis in early-stage non-small cell lung cancer, we conducted proteomic analysis of tissue that had been completely resected. We selected sixteen patients whose tumors were pathological stage I adenocarcinoma with
Roberta Cozzi et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 26(5), 2008-2018 (2012-01-19)
Group B Streptococcus pili are covalently linked structures assembled via a sortase-catalyzed transpeptidation mechanism involving specific residues and motifs. A sequence element containing a conserved glutamic acid, called the E-box, has been described to be involved in pilus formation. Although
Alberto Nuñez et al.
Journal of agricultural and food chemistry, 57(22), 10951-10958 (2009-10-29)
Several studies have suggested that the emulsification properties associated with pectin obtained from sugar beet (Beta vulgaris) are due to the presence of a protein-pectin complex. Nevertheless, the identity of the protein has remained elusive. Pectin, extracted from sugar beet
Joungil Choi et al.
The Journal of biological chemistry, 281(16), 10816-10824 (2006-03-07)
Mutations in DJ-1 cause an autosomal recessive, early onset familial form of Parkinson disease (PD). However, little is presently known about the role of DJ-1 in the more common sporadic form of PD and in other age-related neurodegenerative diseases, such

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