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G0376

Sigma-Aldrich

β-Glucuronidase from bovine liver

Type B-3, ≥2,000,000 units/g solid

Sinônimo(s):

β-D-Glucuronide glucuronosohydrolase

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About This Item

Número CAS:
9001-45-0
Número da licença da enzima:
3.2.1.31 (BRENDA, IUBMB)
Número CE:
232-606-8
Número MDL:
MFCD00130629
Código UNSPSC:
12352204
NACRES:
NA.54

fonte biológica

bovine liver

Nível de qualidade

200

tipo

Type B-3

Formulário

solid

atividade específica

≥2,000,000 units/g solid

temperatura de armazenamento

−20°C

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Categorias relacionadas

Aplicação

β-glucuronidase is used as a reporter gene in GUS assays to monitor gene expression.

Ações bioquímicas/fisiológicas

β-glucuronidase catalyzes the breakdown of complex carbohydrates. In humans it converts conjugated bilirubin into the unconjugated form, making bilirubin suitable for reabsorption.

Definição da unidade

One Sigma or modified Fishman unit will liberate 1.0 μg of phenolphthalein from phenolphthalein glucuronide per hr at 37 °C at pH 5.0 (30 min assay).

Pictogramas

Health hazard
GHS08

Palavra indicadora

Danger

Frases de perigo

H317,H334

Classificações de perigo

Resp. Sens. 1 - Skin Sens. 1

Código de classe de armazenamento

11 - Combustible Solids

Classe de risco de água (WGK)

WGK 3

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

Equipamento de proteção individual

Eyeshields, Gloves, type N95 (US)

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Certificados de análise (COA)

Lot/Batch Number
SLBN7101V
SLBH4556V
011M7020
011M7020V
069K7028

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β-Glucuronidase Reporter Gene Staining Kit

Xiaoyan Liu et al.
Zhejiang da xue xue bao. Yi xue ban = Journal of Zhejiang University. Medical sciences, 42(1), 67-74 (2013-03-19)
To construct a lentiviral RNA interference system targeting heparanase (HPSE) based on miR30 and to test its silencing effect. Three heparanase-shRNA structures were designed based miR30. The targeting fragments were obtained by PCR, then inserted into the vector LV PP-GFP
Klotho to treat kidney fibrosis.
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Journal of the American Society of Nephrology : JASN, 24(5), 687-689 (2013-04-20)
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The activity of heparanase is responsible for heparan sulfate cleavage, thus resulting in the release of heparan sulfate-bound growth factors. Since heparanase activity is upregulated in several tumor types and is implicated in the malignant behavior, the enzyme is regarded
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Genes coding for avenin-like proteins (ALP) represent a new family of wheat storage protein genes. To find a wheat endosperm-specific promoter, a 1644-bp fragment upstream of the ALP type-B gene (GenBank accession number JN622144) was isolated. The important promoter elements
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Nature reviews. Molecular cell biology, 14(3), 166-180 (2013-02-14)
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Protocolos

Enzymatic Assay of ß-Glucuronidase (EC 3.2.1.31) from Helix Pomatia and Bovine Liver

Enzymatic Assay of ß-Glucuronidase (EC 3.2.1.31) from Helix Pomatia and Bovine Liver

Using UHPLC/MS (TOF) for Detection of Drugs and Metabolites in Urine Following Optimized Enzymatic Hydrolysis Conditions

To optimize hydrolysis using β-glucuronidase, factors such as incubation time, temperature, hydrolysis pH, enzyme source, and enzyme concentration must be evaluated for each glucuronide metabolite to be analyzed.

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