S1629
Sulfatase from Aerobacter aerogenes
Type VI, buffered aqueous glycerol solution, 2-5 units/mg protein (biuret), 10-20 units/mL
Sinônimo(s):
Aryl-sulfatase, Aryl-sulfate sulfohydrolase, Phenolsulfatase
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About This Item
Número CAS:
Número MDL:
Código UNSPSC:
12352204
NACRES:
NA.54
Produtos recomendados
tipo
Type VI
Nível de qualidade
forma
buffered aqueous glycerol solution
atividade específica
2-5 units/mg protein (biuret)
peso molecular
~41 kDa
concentração
10-20 units/mL
atividade externa
β-Glucuronidase ≤10 U/mL
Condições de expedição
wet ice
temperatura de armazenamento
−20°C
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Descrição geral
Sulfatases comprise Cys/Ser-X-Pro-X-Arg motif that is conserved in their active sites.
Aplicação
Sulfatase from Aerobacter aerogenes has been used:
- as a deconjugation enzyme for treating plasma samples for quercetin quantification using liquid chromatography with tandem mass spectrometry (LC/MS/MS) analyses
- in fluorescence intensity-based enzymatic assay with an activity-based probe probe 1
- to treat sulfatide liposomes to remove the 3-O-sulfogalactosyde head from sulfatides
Ações bioquímicas/fisiológicas
Sulfatases hydrolyze sulfate ester bonds to generate inorganic sulfates. Microbial sulfatases participate in sulfur scavenging and are essential enzymes for the utilization of sulfur. They may be associated with pathogenesis. Commercially available sulfatase from Aerobacter aerogenes is useful as a deconjugation enzyme for the removal of glucuronate and sulfate moieties from conjugates. Sulfatases find application in industry and agriculture.
Definição da unidade
One unit will hydrolyze 1.0 μmole of p-nitrophenyl sulfate per min at pH 7.1 at 37 °C.
forma física
Solution in 50% glycerol containing 0.01 M Tris, pH 7.5.
substrato
Nº do produto
Descrição
Preços
Código de classe de armazenamento
10 - Combustible liquids
Classe de risco de água (WGK)
WGK 2
Ponto de fulgor (°F)
Not applicable
Ponto de fulgor (°C)
Not applicable
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Arylsulfatase activity was detected in a bacterial strain, Citrobacter braakii 69-b, isolated from soil by enrichment cultivation using porcine gastric mucin. The production of arylsulfatase was derepressed markedly in a synthetic medium by the addition of tyramine. The purified enzyme
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