GUSS
β-Glucuronidase Reporter Gene Staining Kit
Sinônimo(s):
β-Glucuronidase Staining Kit, Reporter Gene Kit, Staining Kit for Glucuronidase
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About This Item
Código UNSPSC:
12161503
NACRES:
NA.32
Produtos recomendados
uso
kit sufficient for 100 histochemical assays
Nível de qualidade
técnica(s)
microbe id | staining: suitable
Condições de expedição
dry ice
temperatura de armazenamento
−20°C
Descrição geral
Kit is used to anaylze β glucuronidase (E.coli GUS gene) expression in transformed plants.
Aplicação
The E. coli GUS gene is extensively used to analyze gene expression in transformed plants. Plants have low intrinsic GUS activity, and the E. coli gene is quite stable in plant cells. As a tag, GUS remains active at the N-terminus of fusion proteins.
Kit is suitable for biochemical activity assays, immunological assays and histochemical staining of tissue and cells.
Kit is suitable for biochemical activity assays, immunological assays and histochemical staining of tissue and cells.
Características e benefícios
- Histochemical staining of plant tissues expressing the E. coli GUS enzyme
- Ideally suited to plant expression studies due to very low GUS activity in plants and high enzyme stability
- GUS does not interfere with plant cell function or viability
Outras notas
The substrate used in this kit is X-GlcA (5-bromo-4-chloro-3-indolyl β-D-glucuronide) which results in an insoluble indigo-blue precipitate in transfected cells and tissues.
Somente componentes do kit
Nº do produto
Descrição
- 2× Fixation Buffer 25 mL
- 5-Bromo-4-chloro-3-indolyl β-D-glucuronide cyclohexylammonium salt, ≥98% 1 mL/vial
- Reagent A for gus staining kit 50 mL
- Reagent B for gus staining kit 200 μL
- Reagent C for gus staining kit 200 μL
Frases de perigo
Declarações de precaução
Classificações de perigo
Aquatic Chronic 3
Código de classe de armazenamento
11 - Combustible Solids
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The GUS reporter gene system is already a powerful tool for the assessment of gene activity in transgenic plants. Further developments may lead to routine in vivo analysis and fusion genetics.
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