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E7885

Sigma-Aldrich

Elastase from porcine pancreas

lyophilized powder, suitable for cell culture

Sinônimo(s):

Elastase from hog pancreas, Pancreatopeptidase E

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About This Item

Número CAS:
39445-21-1
Número da licença da enzima:
3.4.21.36 (BRENDA, IUBMB)
Número CE:
254-453-6
Número MDL:
MFCD00130998
Código UNSPSC:
12352204
NACRES:
NA.75

fonte biológica

Porcine

Nível de qualidade

200

forma

lyophilized powder

atividade específica

≥4 units/mg protein

peso molecular

25.9 kDa

embalagem

pkg of 1 μmol

técnica(s)

cell culture | mammalian: suitable

pH

8

adequação

suitable for

atividade externa

Trypsin ≤50 BAEE units/mg protein

Condições de expedição

dry ice

temperatura de armazenamento

−20°C

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Descrição geral

Elastase is a single polypeptide chain of 240 amino acid residues and contains four disulfide bridges. The molecular mass is approximately 25.9kDa. The enzyme is synthesized as an inactive zymogen, proelastase, which is converted to the active form by limited proteolysis at the N-terminal by trypsin.

Aplicação

Elastase from porcine pancreas is used for the following applications:
  • Cell culture techniques
  • ELISA techniques
  • Used in assay for elastase inhibitory activity

Ações bioquímicas/fisiológicas

Elastase hydrolyses elastin, the specific protein of elastic fibers, and digests hemoglobin, casein and fibrin.
Enzyme that degrades elastin, a matrix component of tissues involved in stretching processes. It is a serine protease with broad specificity. It cleaves protein at the carboxyl side of small hydrophobic amino acids such as Ile, Gly, Ala, Ser, Val, and Leu. The enzyme also hydrolyzes amides and esters such as N-Benzoyl-L-alanine methyl ester.

Embalagem

Package size based on protein content

Definição da unidade

One unit will hydrolyze 1.0 μmole of N-succinyl-L-Ala-Ala-Ala-p-nitroanilide per min, pH 8.0 at 25 °C.

Aplicação

Nº do produto
Descrição
Preços

inibidor

Nº do produto
Descrição
Preços

substrato

Nº do produto
Descrição
Preços

Pictogramas

Health hazardExclamation mark
GHS08,GHS07

Palavra indicadora

Danger

Frases de perigo

H315,H319,H334,H335

Classificações de perigo

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

Órgãos-alvo

Respiratory system

Código de classe de armazenamento

11 - Combustible Solids

Classe de risco de água (WGK)

WGK 3

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

Certificados de análise (COA)

Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.

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Sigma-Aldrich

T3824

Tirosinase

N-Succinil-Ala-Ala-Pro-Fen p-nitroanilida

Sigma-Aldrich

S7388

N-Succinil-Ala-Ala-Pro-Fen p-nitroanilida

N-Succinyl-Ala-Ala-Val-Ala p-nitroanilide protease substrate

Sigma-Aldrich

S7632

N-Succinyl-Ala-Ala-Val-Ala p-nitroanilide

P Hamet et al.
The Journal of biological chemistry, 264(21), 12364-12369 (1989-07-25)
The induction of cyclic GMP formation in target tissues, i.e. vascular smooth muscle and endothelial cells, by atrial natriuretic factor is followed by its egression into plasma and urine. Since the extracellular appearance of this cyclic nucleotide is used as
F Seibold et al.
Gut, 32(10), 1192-1197 (1991-10-01)
The presence of antibodies against pancreatic juice (PAB) in patients with Crohn's disease has recently been reported. In our study sera from 273 patients with inflammatory bowel disease (222 with Crohn's disease, 51 with ulcerative colitis) have been examined for
Yoshiyuki Okumura et al.
Nihon Ishinkin Gakkai zasshi = Japanese journal of medical mycology, 48(1), 13-18 (2007-02-09)
The amino acid sequence of elastase inhibitor, AFLEI, isolated from Aspergillus flavus was determined by the Edman sequencing procedure of peptides derived from digests utilizing clostripain. A molecular weight of 7,525.8 was observed by TOF-MS. AFLEI contained 68 amino acid
Adrien Lupieri et al.
Cardiovascular research, 116(2), 438-449 (2019-05-21)
Defects in efficient endothelial healing have been associated with complication of atherosclerosis such as post-angioplasty neoatherosclerosis and plaque erosion leading to thrombus formation. However, current preventive strategies do not consider re-endothelialization in their design. Here, we investigate mechanisms linking immune
Hao Li et al.
Nature communications, 11(1), 3218-3218 (2020-06-27)
Unruptured intracranial aneurysm (UIA) is a life-threatening cerebrovascular condition. Whether changes in gut microbial composition participate in the development of UIAs remains largely unknown. We perform a case-control metagenome-wide association study in two cohorts of Chinese UIA patients and control
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Protocolos

Enzymatic Assay of Elastase (EC 3.4.21.36)

Enzymatic Assay of Elastase (EC 3.4.21.36), a continuous spectrophotometric rate determination.

Chromatograms

application for HPLC

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