GCによる焙煎したコーヒー豆の分析、Omegawax 250、SPME処理、50/30 μm DVB/Carboxen/PDMS使用
材料
SPMEファイバー
SPMEファイバーアセンブリー ジビニルベンゼン/カルボキセン/ポリジメチルシロキサン (DVB/CAR/PDMS)
needle size 24 ga, StableFlex, for use with autosampler分析カラム
標準
移動相成分
CONDITIONS
sample/matrix
2 g of coffee beans
SPME fiber
50/30 μm divinylbenzene/Carboxen on polydimethylsiloxane on a StableFlex fiber (57329-U)
extraction
headspace, with a pre-equilibration period of 10 min. at 60 °C, and then a fiber exposure of 40 min. at 60 °C, agitated in an alternate clockwise-anticlockwise rotation mode at 500 rpm
desorption process
260 °C for 5 min
column
Omegawax 250, 30 m x 0.25 mm I.D., 0.25 μm (24136)
oven
40 °C (5 min), 4 °C/min to 230 °C, 50 °C/min to 280 °C (2 min)
inj. temp.
260 °C
detector
MS, m/z 40-400
carrier gas
helium, 34.8 cm/sec constant
injection
splitless
scan range
m/z 40-400
sample
Roasted coffee
liner
0.75 mm I.D. SPME/Splitless (2633901)
詳細
アナリシスノート
Headspace-SPME followed by GC analysis was used in this application to analyze volatile compounds in roasted coffee. SPME is ideal for analyzing the headspace of solid samples like coffee beans. The Carboxen/DVB/PDMS fiber is the fiber of choice for analyzing the wide range of analytes commonly found in roast coffee beans.The relatively polar Omegawax 250 GC column provided high resolution.
法的情報
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