V2258

モノクロナール抗ビメンチン マウス宿主抗体

clone LN-6, ascites fluid

• 別名: Anti-F5H288
• MDL番号: MFCD00162904
• UNSPSCコード: 12352203
• NACRES: NA.41

特性

• 由来生物: mouse
• 結合体: unconjugated
• 抗体製品の状態: ascites fluid
• 抗体製品タイプ: primary antibodies
• クローン: LN-6, monoclonal
• 含みます: 15 mM sodium azide
• 化学種の反応性: pig, mouse, sheep, human, bovine, rabbit, feline, rat
• テクニック: immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:200 using human tissue sections; immunoprecipitation (IP): suitable; indirect immunofluorescence: suitable using cultured cells; western blot: suitable
• アイソタイプ: IgM
• UniProtアクセッション番号: P08670
• 輸送温度: dry ice
• 保管温度: −20°C
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... VIM(7431) ; mouse ... Vim(22352) ; rat ... Vim(81818)

詳細

詳細

Vimentin belongs to the class of type III intermediate filament protein family. The gene is located on human chromosome 10p13. It is a cytoskeletal protein. Vimentin is mainly expressed in normal mesenchymal cells.

Vimentin is a 58 kD class III intermediate filament present mainly in mesenchymal cells. It regulates cell shape, motility and endurance of mechanical stress in mesenchymal cells. Monoclonal anti-vimentin antibody can be used for the localization of vimentin by various immunochemical assays like immunoblotting, immunohistochemistry and immunoprecipitation. Frozen B5 fixed and formalin fixed, paraffin embedded tissue sections can be stained using anti-vimentin antibody. Mouse monoclonal anti-vimentin antibody reacts specifically with an epitope of the cytoskeletal protein vimentin (58kD). The product shows cross reactivity with vimentin from bovine, human, pig, sheep, cat, rat, mouse and rabbit.

特異性

細胞骨格タンパク質ビメンチンのエピト-プを認識しますが、ヒトリンパ球系細胞またはリンパ球由来悪性腫瘍は検出しません。このことから、肉腫とリンパ種を識別する際に有効です。

免疫原

ヒト胸腺核抽出物。

アプリケーション

Monoclonal Anti-Vimentin antibody can be used in immunocytochemistry and immunohistochemical staining. It can also be used in immunofluorescence staining (diluted 1: 500) .

Monoclonal Anti-Vimentin antibody produced in mouse has been used in western blotting and immunolabeling.

生物化学的／生理学的作用

Vimentin plays an important role in modulating cell growth. It plays a pivotal role in epithelial-mesenchymal transition. Vimentin acts as a potential target for anti-metastasis therapy for nasopharyngeal carcinoma (NPC). It regulates cellular architecture and tissue integrity. It is also involved in tumorigenesis and the metastatic spread of cancer. Vimentin provides stress resistance. It might be involved in neuritogenesis.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: nwg
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable