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Merck

SAB4200761

Sigma-Aldrich

Anti-Vimentin antibody, Mouse monoclonal

enhanced validation

clone LN-6, purified from hybridoma cell culture

別名:

Anti-VIM

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About This Item

UNSPSCコード:
12352203
NACRES:
NA.41

由来生物

mouse

品質水準

抗体製品の状態

purified from hybridoma cell culture

抗体製品タイプ

primary antibodies

クローン

LN-6, monoclonal

形状

buffered aqueous solution

分子量

~58 kDa

化学種の反応性

rat, human, porcine, monkey, bovine, canine, mouse, rabbit, hamster

強化検証

independent
Learn more about Antibody Enhanced Validation

濃度

~1 mg/mL

テクニック

flow cytometry: suitable
immunoblotting: 0.25-0.5 μg/mL using human foreskin fibroblast Hs68 cell line.
immunofluorescence: 1-2 μg/mL using human foreskin fibroblast Hs68 cell line.
immunohistochemistry: 5-10 μg/mL using Human breast carcinoma sections.
immunoprecipitation (IP): suitable

アイソタイプ

IgM

UniProtアクセッション番号

輸送温度

dry ice

保管温度

−20°C

ターゲットの翻訳後修飾

unmodified

遺伝子情報

bovine ... Vim(280955)
dog ... Vim(477991)
hamster ... Vim(100689099)
human ... VIM(7431)
mouse ... Vim(22352)
pig ... Vim(100522394)
rabbit ... Vim(100008924)
rat ... Vim(81818)
rhesus monkey ... Vim(705289)

詳細

Anti-Vimentin antibody, Mouse monoclonal (mouse IgM isotype) is derived from the LN-6 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from mouse. Vimentin protein is localized in a wide range of cell types, including pancreatic precursor cells, sertoli cells, mesangial cells, trophoblastic giant cells, neuronal precursor cells, fibroblasts, macrophages, endothelial cells of lung blood vessels, renal tubular cells, neutrophils, leukocytes and renal stromal cells.
Vimentin is a specific type III intermediate filament (IF) protein. It is abundantly expressed in mesenchymal cells. The gene encoding vimentin is located on human chromosome 10p13.

特異性

Anti-Vimentin antibody, Mouse monoclonal specifically recognizes a unique epitope of Vimentin protein which is not expressed in cells of hematopoetic derivation. The antibody is reactive with benign and malignant tumors of mesenchymal and neural derivation but does not react with normal and malignanat human lymphoid cells or derived malignancies. The antibody reacts with Vimentin from human, hamster, mouse, rat, canine, monkey, bovine, porcine and rabbit origin.

免疫原

Human thymic nuclear extract

アプリケーション

Anti-Vimentin antibody, Mouse monoclonal may be used in:
  • immunoblotting
  • immunofluorescence
  • flow cytometry
  • immunoprecipitation
  • immunohistochemistry

生物化学的/生理学的作用

Vimentin helps to preserve the structure of cells and stability of tissue in mesenchymal cells. It is involved in tumorigenesis, EMT (epithelial mesenchymal transition) and the metastatic of cancer. It also controls infectious internalization of human papillomavirus 16 pseudovirions.
Vimentin protein plays a significant role in supporting and anchoring the cellular position of organelles such as the nucleus, endoplasmic reticulum, mitochondria, maintenance of cell shape, the endurance of mechanical stress of mesenchymal cells and motility. Overexpression of the vimentin gene is observed in various epithelial cancers, including prostate, gastrointestinal tumors, central nervous system (CNS) tumors, breast, malignant melanoma and lung cancer.

物理的形状

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide

保管および安定性

For continuous use, store at 2–8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

免責事項

Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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保管分類コード

10 - Combustible liquids

WGK

WGK 3

引火点(°F)

Not applicable

引火点(℃)

Not applicable


試験成績書(COA)

製品のロット番号・バッチ番号を入力して、試験成績書(COA) を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。

以前この製品を購入いただいたことがある場合

文書ライブラリで、最近購入した製品の文書を検索できます。

文書ライブラリにアクセスする

Daying Dai et al.
AJNR. American journal of neuroradiology, 26(10), 2560-2568 (2005-11-16)
The purpose of this study was to clarify the cellular mechanisms of aneurysmal healing by comparing histologic and immunohistochemical findings in experimental rabbit and swine aneurysms to a human aneurysm embolized with platinum coils. Swine sidewall aneurysms (n = 5
Vimentin modulates infectious internalisation of HPV16 pseudovirions.
Schafer G, et al.
Journal of Virology, 91(16), e00307-e00317 (2017)
Audrey Gilson et al.
Arthritis research & therapy, 12(1), R24-R24 (2010-02-16)
Development of cell therapies for repairing the intervertebral disc is limited by the lack of a source of healthy human disc cells. Stem cells, particularly mesenchymal stem cells, are seen as a potential source but differentiation strategies are limited by
The gene encoding human vimentin is located on the short arm of chromosome 10.
Ferrari S E R G I O, et al.
American Journal of Human Genetics, 41(4), 616-616 (1987)
Jui M Dave et al.
Microcirculation (New York, N.Y. : 1994), 21(4), 333-344 (2014-01-07)
Angiogenesis is a multistep process that requires intricate changes in cell shape to generate new blood vessels. IF are a large family of proteins that play an important structural and functional role in forming and regulating the cytoskeleton. Vimentin, a

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