P6282

Poly-ʟ-Lysine Hydrobromide

synthetic, mol wt 70,000-150,000, powder, γ-irradiated, suitable for cell culture, BioXtra

• Sinônimo(s): PLL HBr
• Número CAS: 25988-63-0
• Número MDL: MFCD00237230
• Código UNSPSC: 12352202
• eCl@ss: 32160406
• ID de substância PubChem: 24898753
• NACRES: NA.75

Propriedades

• product name: Poli-L-lisina, mol wt 70,000-150,000, lyophilized powder, γ-irradiated, BioXtra, suitable for cell culture
• fonte biológica: synthetic (organic)
• Nível de qualidade: 200
• esterilidade: γ-irradiated
• linha de produto: BioXtra
• forma: lyophilized powder
• peso molecular: 70,000-150,000
• embalagem: pkg of 5 mg
• técnica(s): cell culture | mammalian: suitable
• cobertura de superfície: 4 μg/cm²
• solubilidade: H₂O: soluble 50 mL/vial, clear, colorless
• Condições de expedição: ambient
• temperatura de armazenamento: −20°C
• cadeia de caracteres SMILES: Cl.NCCCCC(N)C(O)=O
• InChI: 1S/C18H38N6O4/c19-10-4-1-7-13(22)16(25)23-14(8-2-5-11-20)17(26)24-15(18(27)28)9-3-6-12-21/h13-15H,1-12,19-22H2,(H,23,25)(H,24,26)(H,27,28)/t13-,14-,15-/m0/s1
• chave InChI: WBSCNDJQPKSPII-KKUMJFAQSA-N

Descrição

Descrição geral

Poly-L-lysine is a positively charged amino acid polymer with approximately one HBr per lysine residue. The hydrobromide allows the poly-L-lysine to be in a crystalline form soluble in water. A small amount of product may be found in the beta structure because the HBr interferes with hydrogen bonding between amino and either the carboxyl groups or N or O containing moieties.

Aplicação

Poly-L-lysine hydrobromide has been used to precoat glass bottom dishes used for in vitro cell culture.

Recommended as a cell culture substratum when using 0.5 ml of a 0.1 mg/ml solution to coat 25 cm². Optimal conditions for attachment must be determined for each cell line and application.

Ações bioquímicas/fisiológicas

Poly-L-lysine serves as a pre-coating substance to ensure better attachment of cells to culture surfaces. Research shows that plates coated with poly-L-lysine (PLL) demonstrated an accelerated cell growth rate. Culturing mesenchymal stem cells (MSCs) on PLL-coated substrates has been found to safeguard the stemness properties of these cells and inhibit senescence. PLL-coated plates have demonstrated enhanced MSC growth and up-regulated the expression of genes associated with cell adhesion, differentiation, proliferation, and signaling. Moreover, porous PLL-coated surfaces have shown improvements in cell proliferation and the osteogenic differentiation of MSCs.

Componentes

A poli-L-lisina é um polímero de aminoácido com carga positiva, com cerca de um HBr por resíduo de lisina. O hidrobrometo possibilita que a poli-L-lisina esteja em uma forma cristalina solúvel em água. Uma pequena quantidade do produto pode ser encontrada na estrutura beta, porque o HBr interfere na ligação de hidrogênio entre o grupo amina e o grupo carboxila ou as porções que contêm N ou O.

Atenção

As soluções estéreis são estáveis por até 2 anos se armazenadas a 2 ºC a 8 °C. Deve ser armazenado dessecado a -20 °C.

Nota de análise

Molecular weight based on viscosity. Also assayed by MALLS.

Outras notas

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Informações de segurança

• Código de classe de armazenamento: 11 - Combustible Solids
• Classe de risco de água (WGK): WGK 3
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable
• Equipamento de proteção individual: Eyeshields, Gloves, type N95 (US)