P1274

Poli-L-lisina

suitable for cell culture, Mol wt 70,000-150,000 by viscosity

• Sinônimo(s): L-Lysine homopolymer hydrobromide
• Número CAS: 25988-63-0
• Número MDL: MFCD00237230
• Código UNSPSC: 12352209
• ID de substância PubChem: 24898180
• NACRES: NA.26

Propriedades

• product name: Poli-L-lisina, mol wt 70,000-150,000 by viscosity
• forma: solid
• Nível de qualidade: 300
• peso molecular: 70,000-150,000 by viscosity
• técnica(s): cell culture | mammalian: suitable
• cor: white to off-white
• temperatura de armazenamento: −20°C
• cadeia de caracteres SMILES: Cl.NCCCCC(N)C(O)=O
• InChI: 1S/C18H38N6O4/c19-10-4-1-7-13(22)16(25)23-14(8-2-5-11-20)17(26)24-15(18(27)28)9-3-6-12-21/h13-15H,1-12,19-22H2,(H,23,25)(H,24,26)(H,27,28)/t13-,14-,15-/m0/s1
• chave InChI: WBSCNDJQPKSPII-KKUMJFAQSA-N

Descrição

Aplicação

Poly-L-lysine polymers can be used in promoting cell adhesion to solid substrates, conjugation to methotrexate for increased drug transport, microencapsulation of islets, cell microencapsulation technology, microarray glass slide coating, and chromosomal preparations. Lower molecular weight poly-L-lysine (30,000-70,000) is less viscuous in solution, but higher molecular weight versions provide more attachment sites per molecule.

Poly-L-lysine hydrobromide is used for the following applications:

• Used for some experiments with REF-52 cells, for coating
• For electrophysiological experiments, cells were seeded on poly-L-lysine hydrobromide coated or uncoated cover-slips
• Cell culture of rat neurons
• Indirect binding of monoclonal antibody via poly-llysine

Ações bioquímicas/fisiológicas

Poly-L-lysine is a nonspecific attachment factor for cells useful in promoting cell adhesion to solid substrates by enhancing electrostatic interaction between negatively charged ions of the cell membrane and the culture surface. When it is absorbed to the cell culture surface, poly-L-lysine functions to increase the number of positively charged sites available for cell binding. With cells that can digest poly-L-lysine, poly-D-lysine should be used as the attachment factor.

Componentes

A poli-L-lisina é um polímero de aminoácido com carga positiva, com cerca de um HBr por resíduo de lisina. O hidrobrometo possibilita que a poli-L-lisina esteja em uma forma cristalina solúvel em água. Uma pequena quantidade do produto pode ser encontrada na estrutura beta, porque o HBr interfere na ligação de hidrogênio entre o grupo amina e o grupo carboxila ou as porções que contêm N ou O.

Atenção

As soluções estéreis são estáveis por até 2 anos se armazenadas a 2 ºC a 8 °C. Deve ser armazenado dessecado a -20 °C.

Nota de preparo

This product has a molecular weight of 70,000-150,000. To remove the HBr, dissolve this product in a neutral buffer and dialyze to remove the salts. None of the poly-L-lysine products have been exposed to trifluoroacetic acid and are dialyzed to remove any monomers, dimmers, or trimers, confirmed by thin layer chromatography. In general, to use this product as an attachment factor, add 50 mL of sterile tissue culture grade water to 5 mg of poly-lysine, and aseptically coat the surface with 1 mL per 25 cm² of solution. After 5 minutes, remove the solution through aspiration and thoroughly rinse the surface. Let dry for two hours before introducing cells and medium.

Nota de análise

Molecular weight based on viscosity and is also assayed by MALLS.

Informações de segurança

• Código de classe de armazenamento: 11 - Combustible Solids
• Classe de risco de água (WGK): WGK 3
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable
• Equipamento de proteção individual: Eyeshields, Gloves, type N95 (US)