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Key Documents

L2005

Sigma-Aldrich

Lactoperoxidase from bovine milk

lyophilized powder (essentially salt-free), ≥200 units/mg protein

Sinônimo(s):

Donor:hydrogen peroxide oxidoreductase, Peroxidase

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About This Item

Número CAS:
Número da licença da enzima:
Número CE:
Número MDL:
Código UNSPSC:
12352204
NACRES:
NA.54

fonte biológica

bovine milk

Nível de qualidade

forma

lyophilized powder (essentially salt-free)

atividade específica

≥200 units/mg protein

razão de absorbância

A412/280 nm 0.7-0.9

nº de adesão UniProt

temperatura de armazenamento

−20°C

InChI

1S/H2O3/c1-3-2/h1-2H

chave InChI

JSPLKZUTYZBBKA-UHFFFAOYSA-N

Informações sobre genes

cow ... LPO(280844)

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Descrição geral

Lactoperoxidase is a major enzyme present in bovine milk and belongs to the peroxidase family. It is present in both plants and animals. The enzyme structure comprises a single polypeptide chain made up of 612 amino acid residues.

Aplicação

Lactoperoxidase from bovine milk has been used as a standard for milk lactoperoxidase to study the role of H2O2 in the inhibition of Staphylococcus aureus growth by Lactococcus garvieae in the presence of lactoperoxidase in raw milk. It has also been used in lactoperoxidase-mediated iodination to prepare radiolabeled peptides.

Ações bioquímicas/fisiológicas

Lactoperoxidase contributes to the antimicrobial system of milk by inactivating a wide range of micro-organisms. This lactoperoxidase-mediated antimicrobial system is also identified in human secretions such as tear-fluid, saliva, and milk. Lactoperoxidase catalyzes the oxidation of molecules by releasing H2O2. The product exhibits antimicrobial activity.
Lactoperoxidase catalyzes the oxidation of iodide to iodine by hydrogen peroxide. This activity provides a gentle, specific alternative to chloramine T for the radioiodination of proteins and DNA.

Definição da unidade

One unit will oxidize 1.0 μmole of 2,2′-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) at pH 5.5 at 25 °C.

Nota de análise

Protein determined by Lowry method.

Pictogramas

Health hazard

Palavra indicadora

Danger

Frases de perigo

Declarações de precaução

Classificações de perigo

Resp. Sens. 1

Código de classe de armazenamento

11 - Combustible Solids

Classe de risco de água (WGK)

WGK 1

Equipamento de proteção individual

Eyeshields, Gloves, type N95 (US)


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