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H1506

Sigma-Aldrich

3α-Hydroxysteroid Dehydrogenase from Pseudomonas testosteroni

≥15 units/mg protein

Sinônimo(s):

3α-Hydroxysteroid:NAD(P)+ oxidoreductase

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About This Item

Número CAS:
9028-56-2
Número da licença da enzima:
1.1.1.50 (BRENDA, IUBMB)
Número CE:
232-839-5
Número MDL:
MFCD00130298
Código UNSPSC:
12352204
NACRES:
NA.54

fonte biológica

bacterial (Pseudomonas testosteroni)

Nível de qualidade

200

Formulário

lyophilized solid

atividade específica

≥15 units/mg protein

purificado por

chromatography

composição

Protein, ≥25%

atividade externa

β-hydroxysteroid dehydrogenase ≤0.5%
alcohol dehydrogenase ≤0.05%

temperatura de armazenamento

−20°C

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Aplicação

3α-Hydroxysteroid Dehydrogenase from Pseudomonas testosterone has been used:
  • in the preparation of composite electrode platform to for the determination of androsterone
  • in potassium phosphate buffer for the bioluminescent assay of bile acids in serum
  • for total bile salts assay using an enzymatic method

Ações bioquímicas/fisiológicas

3α-hydroxysteroid dehydrogenase (3α-HSD) may help to modulate the occupancy of the androgen receptor (AR) in androgen target tissues. It may also act in hindering androgen action.

Definição da unidade

One unit will oxidize 1.0 μmole of androsterone per min at pH 8.9 at 25 °C in the presence of β-NAD+.

forma física

Lyophilized powder containing potassium phosphate buffer salt, EDTA, and DL-dithiothreitol

Código de classe de armazenamento

11 - Combustible Solids

Classe de risco de água (WGK)

WGK 3

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

Equipamento de proteção individual

Eyeshields, Gloves, type N95 (US)

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Certificados de análise (COA)

Lot/Batch Number
SLCB6953
SLBQ8675V
SLBN7037V
SLBD1154V
SLBC0623V

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Sigma-Aldrich

221368

Sodium pyrophosphate decahydrate

β-Dinucleotídeo de nicotinamida adenina Grade I, free acid

Roche

NAD100-RO

β-Dinucleotídeo de nicotinamida adenina

Peroxidase Type VI, essentially salt-free, lyophilized powder, ≥250 units/mg solid (using pyrogallol)

Sigma-Aldrich

P8375

Peroxidase

Bin Li et al.
Proteins, 71(2), 670-683 (2007-11-03)
Experimentally determined protein tertiary structures are rapidly accumulating in a database, partly due to the structural genomics projects. Included are proteins of unknown function, whose function has not been investigated by experiments and was not able to be predicted by
Expression and characterization of recombinant type 2 3alpha-hydroxysteroid dehydrogenase (HSD) from human prostate: demonstration of bifunctional 3alpha17beta-HSD activity and cellular distribution
Lin HK, et al.
Molecular Endocrinology, 11(13), 1971-1984 (1997)
Chiaki Hidai et al.
Bioprocess and biosystems engineering, 32(5), 569-573 (2008-11-20)
Developing methods that result in targeting of therapeutic molecules in gene therapies to target tissues has importance, as targeting can increase efficacy and decrease off target-side-effects. Work from my laboratory previously showed that the extracellular matrix protein Del1 is organized
Brandon Bartling et al.
The Analyst, 134(5), 973-979 (2009-04-22)
An electrochemical sensor, based on thick-film screen-printed Ir/C working and counter electrodes, was developed for the detection of total bile acid concentration in a physiological fluid for potential patient management in patients with liver disease. Current electrochemical methods of detecting
Frank Hoffmann et al.
Journal of biotechnology, 129(1), 131-139 (2007-01-30)
3alpha-Hydroxysteroid dehydrogenase/carbonyl reductase (3alpha-HSD/CR) from Comamonas testosteroni belongs to the short chain dehydrogenase/reductase (SDR) protein superfamily and catalyzes the oxidoreduction of a variety of steroid substrates, including the steroid antibiotic fusidic acid. The enzyme also mediates the carbonyl reduction of
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Protocolos

Enzymatic Assay of 3α-Hydroxysteroid Dehydrogenase with Androsterone (EC 1.1.1.50)

Enzymatic Assay of 3α-Hydroxysteroid Dehydrogenase with Androsterone (EC 1.1.1.50)

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