D2197
Diaphorase from Clostridium kluyveri
recombinant, expressed in E. coli, Animal-component free
Sinônimo(s):
Diaphorase, Lipoamide Dehydrogenase, Lipoyl Dehydrogenase
Faça loginpara ver os preços organizacionais e de contrato
About This Item
Produtos recomendados
recombinante
expressed in E. coli
Nível de qualidade
Formulário
solid
atividade específica
>=30 units/mg protein (biuret)
temperatura de armazenamento
−20°C
Aplicação
Diaphorase from Clostridium kluyveri has been used in lactate dehydrogenase assay in gill cells and impregnated on the strip for formate dehydrogenase assay.
Diaphorase is used in various assays to oxidate β-NADH or β-NADPH in the presence of an electron acceptor. Diaphorase from Clostridium Kluyveri has been used for semiquinone generation during benzoquinone reduction. Product D2197 is animal component free.
Ações bioquímicas/fisiológicas
Diaphorase catalyzes the oxidation of either β-NADH or β-NADPH in the presence of an electron acceptor such as methylene blue or 2,6-dichlorophenolindophenol. Diaphorases which are specific for either β-NADH or β-NADPH are known. The pig heart enzyme of Straub seems to have native diaphorase (β-NADH specific) as well as lipoic and lipoamide dehydrogenase activities. It is reported to be a single protein. However, Massey reports that diaphorase is probably a denatured lipoamide dehydrogenase. Pre-incubation of the pig heart preparation with Cu2+ reduces the lipoamide dehydrogenase activity and proportionately increases the β-NADH diaphorase activity. In our laboratory, we have demonstrated this copper effect to some degree on the pig heart enzyme, but no appreciable effect was observed on the Clostridium kluyveri or torula yeast preparations. The lipoamide dehydrogenase:diaphorase ratio is a measure of the denaturation.
Definição da unidade
One unit of diaphorase will oxidize 1.0 micromole of beta-NADH per minute at pH 7.5 at 25 degrees C, with the corresponding reduction of the appropriate electron acceptor.
forma física
Supplied as a lyophilized powder containing trehalose.
Código de classe de armazenamento
11 - Combustible Solids
Classe de risco de água (WGK)
WGK 3
Ponto de fulgor (°F)
Not applicable
Ponto de fulgor (°C)
Not applicable
Escolha uma das versões mais recentes:
Certificados de análise (COA)
Lot/Batch Number
Não está vendo a versão correta?
Se precisar de uma versão específica, você pode procurar um certificado específico pelo número do lote ou da remessa.
Já possui este produto?
Encontre a documentação dos produtos que você adquiriu recentemente na biblioteca de documentos.
Os clientes também visualizaram
Knut Erik Hovda et al.
Scandinavian journal of clinical and laboratory investigation, 75(7), 610-614 (2015-07-24)
The standard diagnostic approach to methanol poisoning is chromatographic measurement of methanol on centrally placed stationary equipment. Methanol poisoning in places where such equipment is unavailable is thus often not diagnosed. Methanol is metabolized to a toxic metabolite, formate; the
Apor Veres-Székely et al.
International journal of molecular sciences, 23(4) (2022-02-27)
Fibroblasts play a central role in diseases associated with excessive deposition of extracellular matrix (ECM), including idiopathic pulmonary fibrosis. Investigation of different properties of fibroblasts, such as migration, proliferation, and collagen-rich ECM production is unavoidable both in basic research and
Claudio F Gonzalez et al.
The Journal of biological chemistry, 280(24), 22590-22595 (2005-04-21)
Most bacteria contain soluble quinone-reducing flavoenzymes. However, no biological benefit for this activity has previously been demonstrated. ChrR of Pseudomonas putida is one such enzyme that has also been characterized as a chromate reductase; yet we propose that it is
Progress in understanding algal bloom-mediated fish kills: the role of superoxide radicals, phycotoxins and fatty acids
Dorantes-Aranda JJ, et al.
PLoS ONE, 10(7), e0133549-e0133549 (2015)
Stephen J Ralph et al.
Pharmaceutical research, 28(11), 2695-2730 (2011-08-25)
Succinate:quinone reductase (SQR) of Complex II occupies a unique central point in the mitochondrial respiratory system as a major source of electrons driving reactive oxygen species (ROS) production. It is an ideal pharmaceutical target for modulating ROS levels in normal
Nossa equipe de cientistas tem experiência em todas as áreas de pesquisa, incluindo Life Sciences, ciência de materiais, síntese química, cromatografia, química analítica e muitas outras.
Entre em contato com a assistência técnica