NAD100-RO

β-Dinucleotídeo de nicotinamida adenina

Grade I, free acid

• Sinônimo(s): β-DPN, β-NAD, Coenzima 1, Cozimase, DPN, NAD, Nadide, Nucleotídeo de difosfopiridina
• Fórmula empírica (Notação de Hill): C₂₁H₂₇N₇O₁₄P₂ · xH₂O
• Número CAS: 53-84-9
• Peso molecular: 663.43 (anhydrous basis)
• Número MDL: MFCD00036253
• Código UNSPSC: 41106305
• ID de substância PubChem: 329818699

Propriedades

• Ensaio: 100% (dry weight)
• forma: lyophilized
• peso molecular: M_r 663.4
• embalagem: pkg of 1 g (10127965001); pkg of 5 g (10127973001)
• fabricante/nome comercial: Roche
• cor: off-white
• pH: ~3.0 (50 mg/mL in water)
• λmax: 260 nm
• Condições de expedição: wet ice
• temperatura de armazenamento: 2-8°C
• cadeia de caracteres SMILES: NC1=NC=NC2=C1N=CN2.O[C@@H]3[C@@H](COP(O)(OP(OC[C@@H](O4)[C@@H](O)[C@@H](O)[C@H]4[N+]5=CC=CC(C(N)=O)=C5)([O-])=O)=O)OC[C@@H]3O
• InChI: 1S/C21H27N7O14P2/c22-17-12-19(25-7-24-17)28(8-26-12)21-16(32)14(30)11(41-21)6-39-44(36,37)42-43(34,35)38-5-10-13(29)15(31)20(40-10)27-3-1-2-9(4-27)18(23)33/h1-4,7-8,10-11,13-16,20-21,29-32H,5-6H2,(H5-,22,23,24,25,33,34,35,36,37)/t10-,11-,13-,14-,15-,16-,20-,21-/m1/s1
• chave InChI: BAWFJGJZGIEFAR-NNYOXOHSSA-N

Descrição

Descrição geral

Nicotinamide adenine dinucleotide (NAD) is a ubiquitously found electron carrier and a cofactor. NAD⁺ contains an adenylic acid and a nicotinamide-5′-ribonucleotide group linked together by a pyrophosphate moiety. In NAD⁺ complexes, the enzyme-cofactor interactions are highly conserved.

Aplicação

NAD (nicotinamide adenine dinucleotide), Grade I has been used in the kinetic studies of human class IV alcohol dehydrogenase.

NAD has been used in in vitro glycogen synthase kinase 3 (GSK3) deacetylation assay. It has also been used as a component in assay mix for the determination of kinetic characteristics using an enzyme-linked kinetic assay.

Ações bioquímicas/fisiológicas

NAD is also involved in microbial catabolism. β-NAD acts as a substrate for various enzymes in several cellular processes.

The NAD (nicotinamide adenine dinucleotide)/NADH ratio regulates the intracellular redox potential, thereby influencing metabolic reactions in vivo. It works as an electron acceptor.

Aceptor de elétrons

Embalagem

Embalado com base no peso sólido.

Qualidade

Purity: 100% β-NAD based on dry weight (enzymatically, CN-complex and absorbance); A₂₅₀nm - A₂₆₀nm : 0.83 ± 0.02, A₂₈₀nm - A₂₆₀nm : 0.22 ± 0.02
Absence of contaminants: LDH inhibitors are not detectable
Organic solvents: =0.05% (GC)

Especificações

Absorbance coefficients: 17.6 x 10³l x mol^-1 x cm^-1 at 260nm, 5.9 x 10³l x mol^-1 x cm^-1 at 333nm (CN complex)

Nota de preparo

Storage conditions (working solution): 10 mg/ml in aqueous solution, contains at least 99% NAD after 4 weeks at 2 to 8 °C

Outras notas

Esta é a forma comum da NAD.

For life science research only. Not for use in diagnostic procedures.

Informações de segurança

• Código de classe de armazenamento: 13 - Non Combustible Solids
• Classe de risco de água (WGK): WGK 1
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable