A9207
Avidin−Agarose from egg white
aqueous glycerol suspension
Sinônimo(s):
Avidin agarose
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About This Item
Produtos recomendados
fonte biológica
egg white
Nível de qualidade
conjugado
agarose conjugate
Formulário
aqueous glycerol suspension
Matriz
cross-linked 6% beaded agarose
ativação da matriz
epichlorohydrin
ligação da matriz
amino
espaçador de matriz
15 atoms
temperatura de armazenamento
−20°C
Descrição geral
Avidin comprises of 0.05% of total egg white protein. It is a glycosylated protein. It corresponds to a molecular weight of 16.5 kDa and exists as tetramer, which can bind a minimum of three biotin molecules.
Aplicação
Avidin is used for the purification of biotin containing proteins, which can enhance cellular binding and internalization of the antibody (Ab)-functionalized capsules compared with control human immunoglobulin (IgG)-functionalized capsules, suggesting these capsules can specifically interact with cancer cells through antibody/antigen recognition.
Avidin-Agarose from egg white has been used:
- in the precipitation of biotin tagged protein by pull down assay in neuroblastoma cell line (SH-SY5Y )
- in the purification of Lewis(x) sugar binding proteins from Schistosoma mansoni
- in avidin bead assay for purification of labelled biotin
- for capture of glycoproteins from murine cornea samples
Ações bioquímicas/fisiológicas
Avidin finds biotechnological applications due to its high-affinity ligand binding property. Avidin interaction with biotin through its carbohydrate chains is widely exploited in biotechnological applications. In cancer cells, avidin-biotin systems helps in the in vivo localization and drug targeting in tumor cells.
Definição da unidade
One unit will bind 1.0 μg of d-biotin.
forma física
Suspension in 50% glycerol, 0.01 M sodium phosphate, pH 7.0, containing 0.02% sodium azide.
Código de classe de armazenamento
10 - Combustible liquids
Classe de risco de água (WGK)
WGK 3
Ponto de fulgor (°F)
Not applicable
Ponto de fulgor (°C)
Not applicable
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