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Key Documents

S0677

Sigma-Aldrich

Estreptavidina

recombinant, expressed in E. coli, lyophilized powder

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About This Item

Número CAS:
Número MDL:
Código UNSPSC:
12352203
NACRES:
NA.46

recombinante

expressed in E. coli

forma

lyophilized powder

capacidade

4 mol/mol binding capacity (Biotin)

temperatura de armazenamento

−20°C

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Descrição geral

Streptavidin is a biotin-binding protein that is obtained from Streptomyces avidinii. It is resistant to extreme temperature, pH, detergents and proteolytic enzymes. Streptavidin has a molecular weight of 60kDa and exists as a homotetramer.

Aplicação

Streptavidin from Streptomyces avidinii has been used-
  • for blocking of the AFM (atomic force microscopy) tip, during the measurement of unbinding forces in the avidin-biotin system
  • for coupling of anti-HA (hemagglutinin) antibodies during cell free synthesis and assembly of proteins on a biochip
  • for the coating of the gelatin flow phantoms to eventually determine the feasibility of IVUS (intravascular ultrasound) transducer for (biotinylated) microbubble-based drug delivery
  • to immobilize proteins and oligonucleotides

Ações bioquímicas/fisiológicas

Streptavidin is an antibiotic that functions by binding to and depleting the essential vitamin biotin from the surrounding environment. Because of its unique properties, streptavidin has found various applications in biological studies, including immunotherapy, immunoassays, hybridization assays, lymphocyte activation, antigen localization and affinity chromatography.

Definição da unidade

One unit will bind 1.0 μg biotin.

forma física

Lyophilized from 5 mM potassium phosphate buffer

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Código de classe de armazenamento

11 - Combustible Solids

Classe de risco de água (WGK)

WGK 3

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

Equipamento de proteção individual

Eyeshields, Gloves, type N95 (US)


Certificados de análise (COA)

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Visite a Biblioteca de Documentos

Cell-free protein synthesis and assembly on a biochip.
Heyman Y et al
Nature Nanotechnology, 7(6), 374-378 (2012)
Polymer Nanoparticles
Progress in Molecular Biology and Translational Science, 104 (2011)
An IVUS transducer for microbubble therapies.
Kilroy JP et al
IEEE Transactions on Ultrasonics, Ferroelectrics, and Frequency Control, 61(3), 441-449 (2014)
Identification of the Heparan Sulfate Binding Sites in the Cellular Prion Protein*
Richard G
The Journal of Biological Chemistry (2002)
Simplified Paper Format for Detecting HIV Drug Resistance in Clinical Specimens by Oligonucleotide Ligation
Nuttada Panpradist
PLoS ONE (2016)

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